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A sol-gel integrate...
A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen
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- Lee, Sang Wook (author)
- Dongguk University, Seoul,University of Tokyo
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- Lee, Jong Hyun (author)
- Inje University
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- Kwon, Hyuck Gi (author)
- Inje University
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- Laurell, Thomas (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Jeong, Ok Chan (author)
- Inje University
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- Kim, Soyoun (author)
- Dongguk University, Seoul
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(creator_code:org_t)
- 2018-03-10
- 2018
- English 5 s.
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In: Analytical Sciences. - : Springer Science and Business Media LLC. - 0910-6340 .- 1348-2246. ; 34:3, s. 317-321
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Abstract
Subject headings
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- Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
Keyword
- Biomarker discovery
- Macro-structure silicon
- MALDI-TOF mass spectrometry
- PSA
- Sensitive diagnosis
- Sol-gel microarray
Publication and Content Type
- art (subject category)
- ref (subject category)
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