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Proteolytic signatu...
Proteolytic signatures define unique thrombin-derived peptides present in human wound fluid in vivo
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- Saravanan, Rathi (author)
- Nanyang Technological University
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- Adav, Sunil S. (author)
- Nanyang Technological University
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- Choong, Yeu Khai (author)
- Nanyang Technological University
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- Van Der Plas, Mariena J.A. (author)
- Lund University,Lunds universitet,Schmidtchen lab,Forskargrupper vid Lunds universitet,Schmidtchen Lab,Lund University Research Groups
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- Petrlova, Jitka (author)
- Lund University,Lunds universitet,Schmidtchen lab,Forskargrupper vid Lunds universitet,Schmidtchen Lab,Lund University Research Groups
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- Kjellström, Sven (author)
- Lund University,Lunds universitet,CEBMMS PI,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Sze, Siu Kwan (author)
- Nanyang Technological University
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- Schmidtchen, Artur (author)
- Lund University,Lunds universitet,Schmidtchen lab,Forskargrupper vid Lunds universitet,Schmidtchen Lab,Lund University Research Groups,Nanyang Technological University,Bispebjerg Hospital
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(creator_code:org_t)
- 2017-10-13
- 2017
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1
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http://dx.doi.org/10... (free)
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https://www.nature.c...
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Abstract
Subject headings
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- The disease burden of failing skin repair and non-healing ulcers is extensive. There is an unmet need for new diagnostic approaches to better predict healing activity and wound infection. Uncontrolled and excessive protease activity, of endogenous or bacterial origin, has been described as a major contributor to wound healing impairments. Proteolytic peptide patterns could therefore correlate and "report" healing activity and infection. This work describes a proof of principle delineating a strategy by which peptides from a selected protein, human thrombin, are detected and attributed to proteolytic actions. With a particular focus on thrombin-derived C-terminal peptides (TCP), we show that distinct peptide patterns are generated in vitro by the human S1 peptidases human neutrophil elastase and cathepsin G, and the bacterial M4 peptidases Pseudomonas aeruginosa elastase and Staphylococcus aureus aureolysin, respectively. Corresponding peptide sequences were identified in wound fluids from acute and non-healing ulcers, and notably, one peptide, FYT21 (FYTHVFRLKKWIQKVIDQFGE), was only present in wound fluid from non-healing ulcers colonized by P. aeruginosa and S. aureus. Our result is a proof of principle pointing at the possibility of defining peptide biomarkers reporting distinct proteolytic activities, of potential implication for improved diagnosis of wound healing and infection.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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