Reproducible Quantification of Cancer-Associated Proteins in Body Fluids Using Targeted Proteomics

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Fältnamn	Indikatorer	Metadata
000		03801naa a2200433 4500
001		oai:lup.lub.lu.se:b6a78db8-4f29-4631-a467-0cc39a533ef8
003		SwePub
008		160401s2012 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://lup.lub.lu.se/record/29949742 URI
024	7	a https://doi.org/10.1126/scitranslmed.30039892 DOI
040		a (SwePub)lu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Huttenhain, Ruth4 aut
245	1 0	a Reproducible Quantification of Cancer-Associated Proteins in Body Fluids Using Targeted Proteomics
264	1	b American Association for the Advancement of Science (AAAS),c 2012
520		a The rigorous testing of hypotheses on suitable sample cohorts is a major limitation in translational research. This is particularly the case for the validation of protein biomarkers; the lack of accurate, reproducible, and sensitive assays for most proteins has precluded the systematic assessment of hundreds of potential marker proteins described in the literature. Here, we describe a high-throughput method for the development and refinement of selected reaction monitoring (SRM) assays for human proteins. The method was applied to generate such assays for more than 1000 cancer-associated proteins, which are functionally related to candidate cancer driver mutations. We used the assays to determine the detectability of the target proteins in two clinically relevant samples: plasma and urine. One hundred eighty-two proteins were detected in depleted plasma, spanning five orders of magnitude in abundance and reaching below a concentration of 10 ng/ml. The narrower concentration range of proteins in urine allowed the detection of 408 proteins. Moreover, we demonstrate that these SRM assays allow reproducible quantification by monitoring 34 biomarker candidates across 83 patient plasma samples. Through public access to the entire assay library, researchers will be able to target their cancer-associated proteins of interest in any sample type using the detectability information in plasma and urine as a guide. The generated expandable reference map of SRM assays for cancer-associated proteins will be a valuable resource for accelerating and planning biomarker verification studies.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
700	1	a Soste, Martin4 aut
700	1	a Selevsek, Nathalie4 aut
700	1	a Roest, Hannes4 aut
700	1	a Sethi, Atul4 aut
700	1	a Carapito, Christine4 aut
700	1	a Farrah, Terry4 aut
700	1	a Deutsch, Eric W.4 aut
700	1	a Kusebauch, Ulrike4 aut
700	1	a Moritz, Robert L.4 aut
700	1	a Niméus, Emmau Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Bröstcancer Proteogenomik,Forskargrupper vid Lunds universitet,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Breast cancer Proteogenomics,Lund University Research Groups4 aut0 (Swepub:lu)onk-ens
700	1	a Rinner, Oliver4 aut
700	1	a Aebersold, Ruedi4 aut
710	2	a Bröstcancer-genetikb Sektion I4 org
773	0	t Science Translational Medicined : American Association for the Advancement of Science (AAAS)g 4:142, s. 94-142q 4:142<94-142x 1946-6242x 1946-6234
856	4	u http://dx.doi.org/10.1126/scitranslmed.3003989y FULLTEXT
856	4	u https://europepmc.org/articles/pmc3766734?pdf=render
856	4 8	u https://lup.lub.lu.se/record/2994974
856	4 8	u https://doi.org/10.1126/scitranslmed.3003989

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By the author/editor: Huttenhain, Ruth; Soste, Martin; Selevsek, Nathal ...; Roest, Hannes; Sethi, Atul; Carapito, Christ ...; show more...; Farrah, Terry; Deutsch, Eric W.; Kusebauch, Ulrik ...; Moritz, Robert L ...; Niméus, Emma; Rinner, Oliver; Aebersold, Ruedi; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

Articles in the publication: Science Translat ...

By the university: Lund University

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