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Searching for early breast cancer biomarkers by serum protein profiling of pre-diagnostic serum; a nested case-control study
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Opstal-van Winden, Annemieke W. J. (author)
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Krop, Esmeralda J. M. (author)
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- Kåredal, Monica (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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Gast, Marie-Christine W. (author)
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- Lindh, Christian (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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- Jeppsson, Marina (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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- Jönsson, Bo A (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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Grobbee, Diederick E. (author)
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Peeters, Petra H. M. (author)
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Beijnen, Jos H. (author)
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van Gils, Carla H. (author)
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Vermeulen, Roel C. H. (author)
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(creator_code:org_t)
- 2011-08-26
- 2011
- English.
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In: BMC Cancer. - : Springer Science and Business Media LLC. - 1471-2407. ; 11
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Abstract
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- Background: Serum protein profiles have been investigated frequently to discover early biomarkers for breast cancer. So far, these studies used biological samples collected at or after diagnosis. This may limit these studies' value in the search for cancer biomarkers because of the often advanced tumor stage, and consequently risk of reverse causality. We present for the first time pre-diagnostic serum protein profiles in relation to breast cancer, using the Prospect-EPIC (European Prospective Investigation into Cancer and nutrition) cohort. Methods: In a nested case-control design we compared 68 women diagnosed with breast cancer within three years after enrollment, with 68 matched controls for differences in serum protein profiles. All samples were analyzed with SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry). In a subset of 20 case-control pairs, the serum proteome was identified and relatively quantified using isobaric Tags for Relative and Absolute Quantification (iTRAQ) and online two-dimensional nano-liquid chromatography coupled with tandem MS (2D-nanoLC-MS/MS). Results: Two SELDI-TOF MS peaks with m/z 3323 and 8939, which probably represent doubly charged apolipoprotein C-I and C3a des-arginine anaphylatoxin (C3a(desArg)), were higher in pre-diagnostic breast cancer serum (p = 0.02 and p = 0.06, respectively). With 2D-nanoLC-MS/MS, afamin, apolipoprotein E and isoform 1 of inter-alpha trypsin inhibitor heavy chain H4 (ITIH4) were found to be higher in pre-diagnostic breast cancer (p < 0.05), while alpha-2-macroglobulin and ceruloplasmin were lower (p < 0.05). C3a(desArg) and ITIH4 have previously been related to the presence of symptomatic and/or mammographically detectable breast cancer. Conclusions: We show that serum protein profiles are already altered up to three years before breast cancer detection.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Biomarkers
- Breast cancer
- Early diagnosis
- 2D-nanoLC-MS/MS
- Prospective
- Proteomics
- SELDI-TOF MS
Publication and Content Type
- art (subject category)
- ref (subject category)
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BMC Cancer
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- By the author/editor
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Opstal-van Winde ...
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Krop, Esmeralda ...
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Kåredal, Monica
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Gast, Marie-Chri ...
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Lindh, Christian
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Jeppsson, Marina
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show more...
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Jönsson, Bo A
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Grobbee, Diederi ...
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Peeters, Petra H ...
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Beijnen, Jos H.
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van Gils, Carla ...
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Vermeulen, Roel ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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BMC Cancer
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Lund University