Sökning: onr:"swepub:oai:lup.lub.lu.se:b7443ef3-5c9b-48a9-9ebb-e36362c994ec" > A European multicen...
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000 | 07004naa a2200937 4500 | |
001 | oai:lup.lub.lu.se:b7443ef3-5c9b-48a9-9ebb-e36362c994ec | |
003 | SwePub | |
008 | 200203s2020 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:142780735 | |
024 | 7 | a https://lup.lub.lu.se/record/b7443ef3-5c9b-48a9-9ebb-e36362c994ec2 URI |
024 | 7 | a https://doi.org/10.1002/jmv.256592 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1427807352 URI |
040 | a (SwePub)lud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Hayes, A.u University of Oxford4 aut |
245 | 1 0 | a A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts |
264 | c 2020-01-17 | |
264 | 1 | b Wiley,c 2020 |
300 | a 10 s. | |
520 | a Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in-house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV species (EV-A71, echovirus 30, coxsackie A virus 21, and EV-D68), HPeV3, and specificity controls. Reported results from 48 in-house and 15 commercial assays showed 98% detection frequencies of high copy (1000 RNA copies/5 µL) transcripts. In-house assays showed significantly greater detection frequencies of the low copy (10 copies/5 µL) EV and HPeV transcripts (81% and 86%, respectively) compared with commercial assays (56%, 50%; P = 7 × 10−5). EV-specific PCRs showed low cross-reactivity with human rhinovirus C (3 of 42 tests) and infrequent positivity in the negative control (2 of 63 tests). Most or all high copy EV and HPeV controls were successfully typed (88%, 100%) by reference laboratories, but showed reduced effectiveness for low copy controls (41%, 67%). Stabilized RNA transcripts provide an effective, logistically simple and inexpensive reagent for evaluation of diagnostic assay performance. The study provides reassurance of the performance of the many in-house assay formats used across Europe. However, it identified often substantially reduced sensitivities of commercial assays often used as point-of-care tests. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Klinisk laboratoriemedicin0 (SwePub)302232 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Clinical Laboratory Medicine0 (SwePub)302232 hsv//eng |
653 | a enterovirus | |
653 | a enterovirus A71 | |
653 | a parechovirus | |
653 | a PCR | |
653 | a RNA transcripts | |
700 | 1 | a Nguyen, D.u University of Oxford4 aut |
700 | 1 | a Andersson, M.u Oxford University Hospital4 aut |
700 | 1 | a Antón, A.u Vall d'Hebron University Hospital4 aut |
700 | 1 | a Bailly, J. L.u University of Auvergne,Clermont-Ferrand University Hospital4 aut |
700 | 1 | a Beard, S.u Public Health England4 aut |
700 | 1 | a Benschop, K. S.M.u National Institute for Public Health and the Environment (RIVM)4 aut |
700 | 1 | a Berginc, N.u Slovenian National Laboratory of Health, Environment and Food4 aut |
700 | 1 | a Blomqvist, S.u Finnish National Institute for Health and Welfare4 aut |
700 | 1 | a Cunningham, E.u Guy's and St Thomas' NHS Foundation Trust4 aut |
700 | 1 | a Davis, D.u Great Ormond Street Hospital4 aut |
700 | 1 | a Dembinski, J. L.u Norwegian Institute of Public Health4 aut |
700 | 1 | a Diedrich, S.u Robert Koch Institut4 aut |
700 | 1 | a Dudman, S. G.u Norwegian Radium Hospital4 aut |
700 | 1 | a Dyrdak, R.u Karolinska Institutet4 aut |
700 | 1 | a Eltringham, G. J.A.u Freeman Hospital4 aut |
700 | 1 | a Gonzales-Goggia, S.u Public Health England4 aut |
700 | 1 | a Gunson, R.4 aut |
700 | 1 | a Howson-Wells, H. C.u Queen's Medical Centre, Nottingham4 aut |
700 | 1 | a Jääskeläinen, A. J.u Helsinki University Central Hospital4 aut |
700 | 1 | a López-Labrador, F. X.u Carlos III Health Institute,University of Valencia4 aut |
700 | 1 | a Maier, M.u University Hospital Leipzig4 aut |
700 | 1 | a Majumdar, M.u National Institute for Biological Standards and Control4 aut |
700 | 1 | a Midgley, S.u Danish Serum Institute, Copenhagen4 aut |
700 | 1 | a Mirand, A.u Clermont-Ferrand University Hospital4 aut |
700 | 1 | a Morley, U.u University College Dublin4 aut |
700 | 1 | a Nordbø, S. A.u Norwegian University of Science and Technology,St. Olav’s University Hospital4 aut |
700 | 1 | a Oikarinen, S.u University of Tampere4 aut |
700 | 1 | a Osman, H.u Birmingham Heartlands Hospital4 aut |
700 | 1 | a Papa, A.u Aristotle University of Thessaloniki4 aut |
700 | 1 | a Pellegrinelli, L.u University of Milan4 aut |
700 | 1 | a Piralla, A.u Policlinico San Matteo Pavia Fondazione4 aut |
700 | 1 | a Rabella, N.u Hospital de Sant Pau4 aut |
700 | 1 | a Richter, J.u Cyprus Institute of Neurology and Genetics4 aut |
700 | 1 | a Smith, M.u King's College Hospital,University of Milan4 aut |
700 | 1 | a Söderlund Strand, A.u Lund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital4 aut0 (Swepub:lu)mikr-aso |
700 | 1 | a Templeton, K.u Royal Infirmary of Edinburgh4 aut |
700 | 1 | a Vipond, B.u Southmead Hospital4 aut |
700 | 1 | a Vuorinen, T.u Turku University Hospital4 aut |
700 | 1 | a Williams, C.u Royal Oldham Hospital4 aut |
700 | 1 | a Wollants, E.u Catholic University of Leuven4 aut |
700 | 1 | a Zakikhany, K.u Karolinska University Hospital4 aut |
700 | 1 | a Fischer, T. K.u Carlos III Health Institute,Danish Serum Institute, Copenhagen4 aut |
700 | 1 | a Harvala, H.u International Blood Group Reference Laboratory4 aut |
700 | 1 | a Simmonds, P.u University of Oxford4 aut |
710 | 2 | a University of Oxfordb Oxford University Hospital4 org |
773 | 0 | t Journal of Medical Virologyd : Wileyg 92:8, s. 1065-1074q 92:8<1065-1074x 0146-6615x 1096-9071 |
856 | 4 | u http://dx.doi.org/10.1002/jmv.25659x freey FULLTEXT |
856 | 4 | u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/jmv.25659 |
856 | 4 8 | u https://lup.lub.lu.se/record/b7443ef3-5c9b-48a9-9ebb-e36362c994ec |
856 | 4 8 | u https://doi.org/10.1002/jmv.25659 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:142780735 |
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