Search: onr:"swepub:oai:lup.lub.lu.se:bac70cbc-4854-4b7d-ba81-91452a948b42" >
Generation of a con...
Generation of a conditionally null allele of the laminin alpha 1 gene
-
Alpy, F (author)
-
Jivkov, I (author)
-
- Sorokin, Lydia (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
-
show more...
-
Klein, A (author)
-
Arnold, C (author)
-
Huss, Y (author)
-
Kedinger, M (author)
-
Simon-Assmann, R (author)
-
Lefebvre, O (author)
-
show less...
-
(creator_code:org_t)
- 2005
- 2005
- English.
-
In: Genesis: The Journal of Genetics and Development. - : Wiley. - 1526-954X. ; 43:2, s. 59-70
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly expressed. In parallel, Lama1(Delta/Delta) embryos display 1) parietal and visceral endoderm differentiation defects with altered expression of cytokeratin 19 and GATA4, respectively, and 2) an induction of apoptosis. This new mouse model is of particular interest as it will allow time- and tissue-specific inactivation of the Lamal gene in various organs. genesis 43:59-70, 2005.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Reichert's membrane
- development
- apoptosis
- GATA4
- conditional inactivation
- Lama1 genes
- knockout
- basement membrane
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database