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The Catalytic Acid-...
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Teze, DavidTechnical University of Denmark
(author)
The Catalytic Acid-Base in GH109 Resides in a Conserved GGHGG Loop and Allows for Comparable α-Retaining and β-Inverting Activity in an N-Acetylgalactosaminidase from Akkermansia muciniphila
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-02-11
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American Chemical Society (ACS),2020
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11 s.
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LIBRIS-ID:oai:lup.lub.lu.se:bd29e90b-6378-4e06-86fc-09b423b3602b
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https://lup.lub.lu.se/record/bd29e90b-6378-4e06-86fc-09b423b3602bURI
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https://doi.org/10.1021/acscatal.9b04474DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Enzymes active on glycosidic bonds are defined according to the stereochemistry of both substrates and products of the reactions they catalyze. The CAZy classification further assigns these enzymes into sequence-based families sharing a common stereochemistry for substrates (either α- or β-) and products (i.e., inverting or retaining mechanism). Here we describe the N-acetylgalactosaminidases AmGH109A and AmGH109B (i.e., GH109: glycoside hydrolase family 109) from the human gut symbiont Akkermansia muciniphila. Notably, AmGH109A displays α-retaining and β-inverting N-acetylgalactosaminidase activities with comparable efficiencies on natural disaccharides. This dual specificity could provide an advantage in targeting a broader range of host-derived glycans. We rationalize this discovery through bioinformatics, structural, mutational, and computational studies, unveiling a histidine residing in a conserved GGHGG motif as the elusive catalytic acid-base of the GH109 family.
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Shuoker, BasharLund University,Technical University of Denmark
(author)
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Chaberski, Evan KirkTechnical University of Denmark
(author)
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Kunstmann, SonjaTechnical University of Denmark
(author)
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Fredslund, FolmerTechnical University of Denmark(Swepub:lu)maxl-ffl
(author)
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Nielsen, Tine SofieTechnical University of Denmark
(author)
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Stender, Emil G.P.Technical University of Denmark
(author)
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Peters, Günther H.J.Technical University of Denmark
(author)
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Karlsson, Eva NordbergLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-eno
(author)
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Welner, Ditte HededamTechnical University of Denmark
(author)
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Hachem, Maher AbouTechnical University of Denmark(Swepub:lu)biot-mah
(author)
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Technical University of DenmarkLund University
(creator_code:org_t)
Related titles
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In:ACS Catalysis: American Chemical Society (ACS)10:6, s. 3809-38192155-5435
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ACS Catalysis
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Lund University