Identification of the ligand of Pru p 3, a peach LTP

Cubells-Baeza, Nuria (author): Technical University of Madrid,Centre for Plant Biotechnology and Genomics

Gómez-Casado, Cristina (author): Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups

Ramírez-Castillejo, Carmen (author): Centre for Plant Biotechnology and Genomics,Technical University of Madrid

Garrido-Arandia, María (author): Centre for Plant Biotechnology and Genomics,Technical University of Madrid

Pacios, Luis F. (author): Centre for Plant Biotechnology and Genomics,Technical University of Madrid

Díaz-Perales, Araceli (author): Centre for Plant Biotechnology and Genomics,Technical University of Madrid

(creator_code:org_t)

2017-03-15
2017
English 12 s.
In: Plant Molecular Biology. - : Springer Science and Business Media LLC. - 0167-4412 .- 1573-5028. ; 94:1-2, s. 33-44

Related links:: http://dx.doi.org/10...; show more...; http://oa.upm.es/500...; https://lup.lub.lu.s...; https://doi.org/10.1...; show less...

Abstract Subject headings

Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.

By the author/editor: Cubells-Baeza, N ...; Gómez-Casado, Cr ...; Tordesillas, Let ...; Ramírez-Castille ...; Garrido-Arandia, ...; González-Melendi ...; show more...; Herrero, María; Pacios, Luis F.; Díaz-Perales, Ar ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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