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Direct contact betw...
Direct contact between Plasmodium falciparum and human B-cells in a novel co-culture increases parasite growth and affects B-cell growth
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- Reddy, Sreenivasulu B. (author)
- Karolinska Institute
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- Nagy, Noemi (author)
- Karolinska Institutet,Karolinska Institute
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- Rönnberg, Caroline (author)
- Karolinska Institute,Karolinska University Hospital
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- Chiodi, Francesca (author)
- Karolinska Institutet,Karolinska Institute
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- Lugaajju, Allan (author)
- Karolinska Institute,Makerere University
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- Heuts, Frank (author)
- Karolinska Institute
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- Szekely, Laszlo (author)
- Karolinska Institutet,Karolinska Institute
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- Wahlgren, Mats (author)
- Karolinska Institute
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- Persson, Kristina E.M. (author)
- Karolinska Institute,Lund University,Lunds universitet,Malaria och Babesia,Forskargrupper vid Lunds universitet,Malaria and Babesia,Lund University Research Groups,Skåne University Hospital
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(creator_code:org_t)
- 2021-07-05
- 2021
- English.
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In: Malaria Journal. - : Springer Science and Business Media LLC. - 1475-2875. ; 20:1
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Abstract
Subject headings
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- Background: Plasmodium falciparum parasites cause malaria and co-exist in humans together with B-cells for long periods of time. Immunity is only achieved after repeated exposure. There has been a lack of methods to mimic the in vivo co-occurrence, where cells and parasites can be grown together for many days, and it has been difficult with long time in vitro studies. Methods and results: A new method for growing P. falciparum in 5% CO2 with a specially formulated culture medium is described. This knowledge was used to establish the co-culture of live P. falciparum together with human B-cells in vitro for 10 days. The presence of B-cells clearly enhanced parasite growth, but less so when Transwell inserts were used (not allowing passage of cells or merozoites), showing that direct contact is advantageous. B-cells also proliferated more in presence of parasites. Symbiotic parasitic growth was verified using CESS cell-line and it showed similar results, indicating that B-cells are indeed the cells responsible for the effect. In malaria endemic areas, people often have increased levels of atypical memory B-cells in the blood, and in this assay it was demonstrated that when parasites were present there was an increase in the proportion of CD19 + CD20 + CD27 − FCRL4 + B-cells, and a contraction of classical memory B-cells. This effect was most clearly seen when direct contact between B-cells and parasites was allowed. Conclusions: These results demonstrate that P. falciparum and B-cells undoubtedly can affect each other when allowed to multiply together, which is valuable information for future vaccine studies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- B-cell
- Culture
- Human
- Malaria
- Plasmodium falciparum
Publication and Content Type
- art (subject category)
- ref (subject category)
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