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Chromatographic ads...
Chromatographic adsorption of serum albumin and antibody proteins in cryogels with benzyl-quaternary amine ligands.
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Yun, Junxian (author)
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Cheng, Xiuhong (author)
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Ye, Jialei (author)
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Shen, Shaochuan (author)
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Yang, Gensheng (author)
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Yao, Kejian (author)
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- Kirsebom, Harald (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Lin, Dong-Qiang (author)
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Guan, Yi-Xin (author)
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Yao, Shan-Jing (author)
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Journal of Chromatography A. - : Elsevier BV. - 0021-9673. ; 1381, s. 173-183
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
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- The preparation and characterization of mixed-mode adsorbents for a typical separation purpose are of great importance in bioseparation areas. In this work, we prepared a new monolithic cryogel with a combination of ion-exchange and hydrophobic functions by employing benzyl-quaternary amine groups. The fundamental cryogel properties, protein equilibrium adsorption isotherm and chromatographic adsorption in the cryogel were measured experimentally. The results showed that, by using bovine serum album as the model protein, the dual functional cryogel has protein binding capability even in salt solution and the buffer with pH close or below the protein isoelectric point due to both the electrostatic and hydrophobic interactions. A capillary-based adsorption model was developed, which provided satisfied insights of the microstructure, axial dispersion, mass transfer as well as protein adsorption characteristics within the cryogel bed. The chromatographic isolation of bioactive proteins from rabbit blood serum was carried out by the cryogel. Immunoglobulin G antibody with a purity of 98.2% and albumin with a purity of 96.8% were obtained, indicating that the cryogel could be an interesting and promising adsorbent in bioseparation areas.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Yun, Junxian
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Cheng, Xiuhong
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Ye, Jialei
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Shen, Shaochuan
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Yang, Gensheng
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Yao, Kejian
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show more...
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Kirsebom, Harald
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Lin, Dong-Qiang
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Guan, Yi-Xin
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Yao, Shan-Jing
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show less...
- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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Journal of Chrom ...
- By the university
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Lund University