Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model

Sigar, Ira M. (author): Dept Microbiol & Immunol, Chicago Coll Osteopath Med, Midwestern Univ, Downers Grove IL, USA

Schripsema, Justin H. (author): Dept Microbiol & Immunol, Chicago Coll Osteopath Med, Midwestern Univ, Downers Grove IL, USA

Wang, Yibing (author): Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Univ Southampton, Southampton, England

Clarke, Ian N. (author): Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Univ Southampton, Southampton, England

Cutcliffe, Lesley T. (author): Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Univ Southampton, Southampton, England

Seth-Smith, Helena M. B. (author): Wellcome Trust Sanger Inst, Hinxton, England

Bjartling, Carina (author): Lund University,Lunds universitet,Urogynekologi och reproduktionsfarmakologi,Forskargrupper vid Lunds universitet,Urogynaecology and Reproductive Pharmacology,Lund University Research Groups,Dept Obstet & Gynecol, Malmö Univ Hosp, Malmö, Sweden

Unemo, Magnus (author): Region Örebro län,Dept Lab Med, Örebro University Hospital, Örebro, Sweden

Persson, Kenneth (author): Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups,Dept Lab Med, Malmö Univ Hosp, Malmö, Sweden

Ramsey, Kyle H. (author): Dept Microbiol & Immunol, Chicago Coll Osteopath Med, Midwestern Univ, Downers Grove IL, USA

(creator_code:org_t)

Wiley-Blackwell, 2014
2014
English.
In: Pathogens and Disease. - : Wiley-Blackwell. - 2049-632X. ; 70:1, s. 61-69

Related links:: http://dx.doi.org/10...; show more...; https://lup.lub.lu.s...; https://doi.org/10.1...; https://urn.kb.se/re...; show less...

Abstract Subject headings

We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)

By the author/editor: Sigar, Ira M.; Schripsema, Just ...; Wang, Yibing; Clarke, Ian N.; Cutcliffe, Lesle ...; Seth-Smith, Hele ...; show more...; Thomson, Nichola ...; Bjartling, Carin ...; Unemo, Magnus; Persson, Kenneth; Ramsey, Kyle H.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Infectious Medic ...

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