Search: onr:"swepub:oai:lup.lub.lu.se:c990db27-23a8-49af-a01f-8dc954444858" >
Development of a D-...
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering
-
Demeke, Mekonnen M. (author)
-
Dietz, Heiko (author)
-
Li, Yingying (author)
-
show more...
-
Foulquie-Moreno, Maria R. (author)
-
- Mutturi, Sarma (author)
- Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
-
Deprez, Sylvie (author)
-
Den Abt, Tom (author)
-
Bonini, Beatriz M. (author)
-
- Lidén, Gunnar (author)
- Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
-
Dumortier, Francoise (author)
-
Verplaetse, Alex (author)
-
Boles, Eckhard (author)
-
Thevelein, Johan M. (author)
-
show less...
-
(creator_code:org_t)
- 2013-06-21
- 2013
- English.
-
In: Biotechnology for Biofuels. - : Springer Science and Business Media LLC. - 1754-6834. ; 6
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://biotechnolog...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background: The production of bioethanol from lignocellulose hydrolysates requires a robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst. The purpose of the present work was to develop such a strain from a prime industrial yeast strain, Ethanol Red, used for bioethanol production. Results: An expression cassette containing 13 genes including Clostridium phytofermentans XylA, encoding D-xylose isomerase (XI), and enzymes of the pentose phosphate pathway was inserted in two copies in the genome of Ethanol Red. Subsequent EMS mutagenesis, genome shuffling and selection in D-xylose-enriched lignocellulose hydrolysate, followed by multiple rounds of evolutionary engineering in complex medium with D-xylose, gradually established efficient D-xylose fermentation. The best-performing strain, GS1.11-26, showed a maximum specific D-xylose consumption rate of 1.1 g/g DW/h in synthetic medium, with complete attenuation of 35 g/L D-xylose in about 17 h. In separate hydrolysis and fermentation of lignocellulose hydrolysates of Arundo donax (giant reed), spruce and a wheat straw/hay mixture, the maximum specific D-xylose consumption rate was 0.36, 0.23 and 1.1 g/g DW inoculum/h, and the final ethanol titer was 4.2, 3.9 and 5.8% (v/v), respectively. In simultaneous saccharification and fermentation of Arundo hydrolysate, GS1.11-26 produced 32% more ethanol than the parent strain Ethanol Red, due to efficient D-xylose utilization. The high D-xylose fermentation capacity was stable after extended growth in glucose. Cell extracts of strain GS1.11-26 displayed 17-fold higher XI activity compared to the parent strain, but overexpression of XI alone was not enough to establish D-xylose fermentation. The high D-xylose consumption rate was due to synergistic interaction between the high XI activity and one or more mutations in the genome. The GS1.11-26 had a partial respiratory defect causing a reduced aerobic growth rate. Conclusions: An industrial yeast strain for bioethanol production with lignocellulose hydrolysates has been developed in the genetic background of a strain widely used for commercial bioethanol production. The strain uses glucose and D-xylose with high consumption rates and partial cofermentation in various lignocellulose hydrolysates with very high ethanol yield. The GS1.11-26 strain shows highly promising potential for further development of an all-round robust yeast strain for efficient fermentation of various lignocellulose hydrolysates.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering (hsv//eng)
Keyword
- Bioethanol
- Lignocellulose
- D-xylose fermentation
- D-xylose isomerase
- Inhibitor tolerance
- Saccharomyces cerevisiae
- Evolutionary engineering
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Demeke, Mekonnen ...
-
Dietz, Heiko
-
Li, Yingying
-
Foulquie-Moreno, ...
-
Mutturi, Sarma
-
Deprez, Sylvie
-
show more...
-
Den Abt, Tom
-
Bonini, Beatriz ...
-
Lidén, Gunnar
-
Dumortier, Franc ...
-
Verplaetse, Alex
-
Boles, Eckhard
-
Thevelein, Johan ...
-
show less...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Chemical Enginee ...
- Articles in the publication
-
Biotechnology fo ...
- By the university
-
Lund University