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Direction of actin ...
Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration
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- Nordenfelt, Pontus (author)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Marine Biological Laboratory,Harvard Medical School
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- Moore, Travis I (author)
- Harvard Medical School
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- Mehta, Shalin B (author)
- Marine Biological Laboratory
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- Kalappurakkal, Joseph Mathew (author)
- Marine Biological Laboratory
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- Swaminathan, Vinay (author)
- Marine Biological Laboratory
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- Koga, Nobuyasu (author)
- University of Washington
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- Lambert, Talley J (author)
- Harvard Medical School
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- Baker, David (author)
- University of Washington, Seattle
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- Waters, Jennifer C (author)
- Harvard Medical School
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- Oldenbourg, Rudolf (author)
- Marine Biological Laboratory
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- Tani, Tomomi (author)
- Marine Biological Laboratory
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Mayor, Satyajit (author)
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Waterman, Clare M (author)
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Springer, Timothy A (author)
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(creator_code:org_t)
- 2017-12-11
- 2017
- English.
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 8:1
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Abstract
Subject headings
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- Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts a specific orientation dictated by the direction of actin flow on the surface of migrating cells. We insert GFP into the rigid, ligand-binding head of the integrin, model with Rosetta the orientation of GFP and its transition dipole relative to the integrin head, and measure orientation with fluorescence polarization microscopy. Cytoskeleton and ligand-bound integrins orient in the same direction as retrograde actin flow with their cytoskeleton-binding β-subunits tilted by applied force. The measurements demonstrate that intracellular forces can orient cell surface integrins and support a molecular model of integrin activation by cytoskeletal force. Our results place atomic, Å-scale structures of cell surface receptors in the context of functional and cellular, μm-scale measurements.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
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- By the author/editor
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Nordenfelt, Pont ...
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Moore, Travis I
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Mehta, Shalin B
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Kalappurakkal, J ...
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Swaminathan, Vin ...
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Koga, Nobuyasu
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show more...
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Lambert, Talley ...
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Baker, David
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Waters, Jennifer ...
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Oldenbourg, Rudo ...
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Tani, Tomomi
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Mayor, Satyajit
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Waterman, Clare ...
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Springer, Timoth ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Nature Communica ...
- By the university
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Lund University