Search: onr:"swepub:oai:lup.lub.lu.se:d17f119d-5457-4a3a-a338-7fbbee42347e" >
Anterograde deliver...
-
Gustafsson, Elin
(author)
Anterograde delivery of brain-derived neurotrophic factor to striatum via nigral transduction of recombinant adeno-associated virus increases neuronal death but promotes neurogenic response following stroke.
- Article/chapterEnglish2003
Publisher, publication year, extent ...
-
2003-06-23
-
Wiley,2003
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:d17f119d-5457-4a3a-a338-7fbbee42347e
-
https://lup.lub.lu.se/record/115713URI
-
https://doi.org/10.1046/j.1460-9568.2003.02713.xDOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
o explore the role of brain-derived neurotrophic factor for survival and generation of striatal neurons after stroke, recombinant adeno-associated viral vectors carrying brain-derived neurotrophic factor or green fluorescent protein genes were injected into right rat substantia nigra 4–5 weeks prior to 30 min ipsilateral of middle cerebral artery occlusion. The brain-derived neurotrophic factor–recombinant adeno-associated viral transduction markedly increased the production of brain-derived neurotrophic factor protein by nigral cells. Brain-derived neurotrophic factor was transported anterogradely to the striatum and released in biologically active form, as revealed by the hypertrophic response of striatal neuropeptide Y-positive interneurons. Animals transduced with brain-derived neurotrophic factor-recombinant adeno-associated virus also exhibited abnormalities in body posture and movements, including tilted body to the right, choreiform movements of left forelimb and head, and spontaneous, so-called 'barrel' rotation along their long axis. The continuous delivery of brain-derived neurotrophic factor had no effect on the survival of striatal projection neurons after stroke, but exaggerated the loss of cholinergic, and parvalbumin- and neuropeptide Y-positive, γ-aminobutyric acid-ergic interneurons. The high brain-derived neurotrophic factor levels in the animals subjected to stroke also gave rise to an increased number of striatal cells expressing doublecortin, a marker for migrating neuroblasts, and cells double-labelled with the mitotic marker, 5-bromo-2'-deoxyuridine-5'monophosphate, and early neuronal (Hu) or striatal neuronal (Meis2) markers. Our findings indicate that long-term anterograde delivery of high levels of brain-derived neurotrophic factor increases the vulnerability of striatal interneurons to stroke-induced damage. Concomitantly, brain-derived neurotrophic factor potentiates the stroke-induced neurogenic response, at least at early stages.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Andsberg, GunnarLund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine(Swepub:lu)neur-gan
(author)
-
Darsalia, VladimerLund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine(Swepub:lu)neur-vda
(author)
-
Mohapel, PaulLund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine(Swepub:lu)neur-pmo
(author)
-
Mandel, Ronald J.
(author)
-
Kirik, DenizLund University,Lunds universitet,Neurobiologi,Forskargrupper vid Lunds universitet,Neurobiology,Lund University Research Groups(Swepub:lu)mphy-dki
(author)
-
Lindvall, OlleLund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine(Swepub:lu)neur-oli
(author)
-
Kokaia, ZaalLund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine(Swepub:lu)neur-zko
(author)
-
Wallenberg Neurocentrum, LundMedicinska fakulteten
(creator_code:org_t)
Related titles
-
In:European Journal of Neuroscience: Wiley17:12, s. 2667-26781460-95680953-816X
Internet link
Find in a library
To the university's database