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- Bergdahl, Gizem ErtürkLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB (author)
Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
- Article/chapterEnglish2019
Publisher, publication year, extent ...
- 2019-04-24
- Springer Science and Business Media LLC,2019
- 10 s.
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:d299a221-3d2b-4bd1-b5a3-760ef4fc5c5d
- https://lup.lub.lu.se/record/d299a221-3d2b-4bd1-b5a3-760ef4fc5c5dURI
- https://doi.org/10.1007/s12010-019-03006-0DOI
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- Language:English
- Summary in:English
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Notes
- A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Biomedicinsk laboratorievetenskap/teknologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Medical Biotechnology Biomedical Laboratory Science/Technology hsv//eng
- TEKNIK OCH TEKNOLOGIER Medicinteknik Medicinsk laboratorie- och mätteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Medical Engineering Medical Laboratory and Measurements Technologies hsv//eng
- Capacitive sensor
- Degradation
- Enzymatic activity
- Protease
Added entries (persons, corporate bodies, meetings, titles ...)
- Hedström, MartinLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB(Swepub:lu)biot-mhe (author)
- Mattiasson, BoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze Biosystems AB(Swepub:lu)biot-bma (author)
- BioteknikCentrum för tillämpade biovetenskaper (creator_code:org_t)
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- In:Applied Biochemistry and Biotechnology: Springer Science and Business Media LLC189, s. 374-3830273-22891559-0291
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