Search: onr:"swepub:oai:lup.lub.lu.se:d4d502cc-9b6f-4aae-853b-f4c4074cf45e" >
The tetraspanin CD6...
-
Källquist, LindaLund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine
(author)
The tetraspanin CD63 is involved in granule targeting of neutrophil elastase.
- Article/chapterEnglish2008
Publisher, publication year, extent ...
-
American Society of Hematology,2008
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:d4d502cc-9b6f-4aae-853b-f4c4074cf45e
-
https://lup.lub.lu.se/record/1223625URI
-
https://doi.org/10.1182/blood-2007-10-116285DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
Targeting mechanisms of neutrophil elastase (NE) and other luminal proteins stored in myeloperoxidase (MPO)-positive secretory lysosomes/primary granules of neutrophils are unknown. These granules contain an integral membrane protein, CD63 with an adaptor protein-3-dependent granule delivery system. Therefore, we hypothesized that CD63 cooperates in granule delivery of the precursor of NE (proNE). Supporting this hypothesis, an association was demonstrated between CD63 and proNE upon coexpression in COS cells. This also involved augmented cellular retention of proNE requiring intact large extracellular loop of CD63. Furthermore, depletion of CD63 in promyelocytic HL-60 cells with RNA interference or a CD63 mutant caused reduction of cellular NE. However, the proNE steady state level was similar to wild type in CD63-depleted clones making it feasible to examine possible effects of CD63 on NE trafficking. Thus, depletion of CD63 led to reduced processing of proNE into mature NE and reduced constitutive secretion. Furthermore, CD63 -depleted cells showed a lack of morphologically normal granules, but contained MPO-positive cytoplasmic vacuoles with a lack of proNE and NE. Collectively, our data suggest that granule proteins may cooperate in targeting; CD63 can be involved in ER or Golgi export, cellular retention and granule targeting of proNE before storage as mature NE.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Hansson, MarkusLund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)hema-mha
(author)
-
Persson, Ann-MajLund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)efor-amp
(author)
-
Janssen, Hans
(author)
-
Calafat, Jero
(author)
-
Tapper, HansLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)medk-hta
(author)
-
Olsson, IngeLund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)efor-iol
(author)
-
Avdelningen för hematologi och transfusionsmedicinInstitutionen för laboratoriemedicin
(creator_code:org_t)
Related titles
-
In:Blood: American Society of Hematology112, s. 3444-34541528-00200006-4971
Internet link
Find in a library
-
Blood
(Search for host publication in LIBRIS)
To the university's database