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Formation of the alpha 1-microglobulin chromophore in mammalian and insect cells : a novel post-translational mechanism?
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- Åkerström, B (author)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Bratt, T (author)
- Lund University
- Enghild, J J (author)
- (creator_code:org_t)
- 1995
- 1995
- English.
- In: FEBS Letters. - 0014-5793. ; 362:1, s. 4-50
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- alpha 1-Microglobulin is an immunosuppressive plasma protein synthesized by the liver. The isolated protein is yellow-brown, but the hypothetical chromophore has not yet been identified. In this work, it is shown that a human liver cell line, HepG2, grown in a completely synthetic and serum-free medium, secretes alpha 1-microglobulin which is also yellow-brown, suggesting a de novo synthesis of the chromophore by the cells. alpha 1-Microglobulin isolated from the culture medium of insect cells transfected with the gene for rat alpha 1-microglobulin is also yellow-brown, suggesting that the gene carries information about the chromophore. Reduction and alkylation or removal of N- or O-linked carbohydrates by glycosidase treatment did not reduce the colour intensity of the protein. An internal dodecapeptide (amino acid positions 70-81 in human alpha 1-microglobulin) was also yellow-brown. The latter results indicate that the chromophore is linked to the polypeptide. In conclusion, the results suggest that the alpha 1-microglobulin gene carries information activating a post-translational protein modification mechanism which is present in mammalian and insect cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Alkylation
- Alpha-Globulins/chemistry
- Amino Acid Sequence
- Animals
- Cell Line
- Glycosylation
- Humans
- Molecular Sequence Data
- Moths
- Oxidation-Reduction
- Peptides/chemistry
- Pigments, Biological/chemistry
- Protein Processing, Post-Translational
- Tumor Cells, Cultured
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- art (subject category)
- ref (subject category)
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- Enghild, J J
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- FEBS Letters
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- Lund University
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