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- Åkerström, BLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine (author)
Formation of the alpha 1-microglobulin chromophore in mammalian and insect cells : a novel post-translational mechanism?
- Article/chapterEnglish1995
Publisher, publication year, extent ...
- 1995
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:d5125f0b-60f7-4923-ae1d-d25b2c8a7fef
- https://lup.lub.lu.se/record/d5125f0b-60f7-4923-ae1d-d25b2c8a7fefURI
- https://doi.org/10.1016/0014-5793(95)00206-ODOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- alpha 1-Microglobulin is an immunosuppressive plasma protein synthesized by the liver. The isolated protein is yellow-brown, but the hypothetical chromophore has not yet been identified. In this work, it is shown that a human liver cell line, HepG2, grown in a completely synthetic and serum-free medium, secretes alpha 1-microglobulin which is also yellow-brown, suggesting a de novo synthesis of the chromophore by the cells. alpha 1-Microglobulin isolated from the culture medium of insect cells transfected with the gene for rat alpha 1-microglobulin is also yellow-brown, suggesting that the gene carries information about the chromophore. Reduction and alkylation or removal of N- or O-linked carbohydrates by glycosidase treatment did not reduce the colour intensity of the protein. An internal dodecapeptide (amino acid positions 70-81 in human alpha 1-microglobulin) was also yellow-brown. The latter results indicate that the chromophore is linked to the polypeptide. In conclusion, the results suggest that the alpha 1-microglobulin gene carries information activating a post-translational protein modification mechanism which is present in mammalian and insect cells.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
- Alkylation
- Alpha-Globulins/chemistry
- Amino Acid Sequence
- Animals
- Cell Line
- Glycosylation
- Humans
- Molecular Sequence Data
- Moths
- Oxidation-Reduction
- Peptides/chemistry
- Pigments, Biological/chemistry
- Protein Processing, Post-Translational
- Tumor Cells, Cultured
Added entries (persons, corporate bodies, meetings, titles ...)
- Bratt, TLund University (author)
- Enghild, J J (author)
- InfektionsmedicinSektion III (creator_code:org_t)
Related titles
- In:FEBS Letters362:1, s. 4-500014-5793
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