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Characterizing Feat...
Characterizing Features of Human Circulating B Cells Carrying CLL-Like Stereotyped Immunoglobulin Rearrangements
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- Bagnara, Davide (author)
- University of Genoa
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- Colombo, Monica (author)
- Ospedale Policlinico San Martino
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- Reverberi, Daniele (author)
- Ospedale Policlinico San Martino
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- Matis, Serena (author)
- Ospedale Policlinico San Martino
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- Massara, Rosanna (author)
- Ospedale Policlinico San Martino
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- Cardente, Niccolò (author)
- University of Genoa
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- Ubezio, Gianluca (author)
- Ospedale Policlinico San Martino
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- Agostini, Vanessa (author)
- Ospedale Policlinico San Martino
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- Agnelli, Luca (author)
- University of Milan
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- Neri, Antonino (author)
- Regional Agency for Health and Social Care; Reggio Emilia
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- Cardillo, Martina (author)
- Feinstein Institute for Medical Research
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- Vergani, Stefano (author)
- Lund University,Lunds universitet,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine
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- Ghiotto, Fabio (author)
- University of Genoa,Ospedale Policlinico San Martino
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- Mazzarello, Andrea Nicola (author)
- University of Genoa
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- Morabito, Fortunato (author)
- Azienda Ospedaliera di Consenza,Augusta Victoria Hospital
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- Cutrona, Giovanna (author)
- Ospedale Policlinico San Martino
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- Ferrarini, Manlio (author)
- University of Genoa
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- Fais, Franco (author)
- University of Genoa,Ospedale Policlinico San Martino
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(creator_code:org_t)
- 2022-06-23
- 2022
- English.
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In: Frontiers in Oncology. - : Frontiers Media SA. - 2234-943X. ; 12
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Abstract
Subject headings
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- Chronic Lymphocytic Leukemia (CLL) is characterized by the accumulation of monoclonal CD5+ B cells with low surface immunoglobulins (IG). About 40% of CLL clones utilize quasi-identical B cell receptors, defined as stereotyped BCR. CLL-like stereotyped-IG rearrangements are present in normal B cells as a part of the public IG repertoire. In this study, we collected details on the representation and features of CLL-like stereotyped-IG in the IGH repertoire of B-cell subpopulations purified from the peripheral blood of nine healthy donors. The B-cell subpopulations were also fractioned according to the expression of surface CD5 molecules and IG light chain, IGκ and IGλ. IG rearrangements, obtained by high throughput sequencing, were scanned for the presence of CLL-like stereotyped-IG. CLL-like stereotyped-IG did not accumulate preferentially in the CD5+ B cells, nor in specific B-cell subpopulations or the CD5+ cell fraction thereof, and their distribution was not restricted to a single IG light chain type. CLL-like stereotyped-IG shared with the corresponding CLL stereotype rearrangements the IGHV mutational status. Instead, for other features such as IGHV genes and frequency, CLL stereotyped-IGs presented a CLL-like subset specific behavior which could, or could not, be consistent with CLL stereotyped-IGs. Therefore, as opposed to the immuno-phenotype, the features of the CLL stereotyped-IG repertoire suggest a CLL stereotyped subset-specific ontogeny. Overall, these findings suggest that the immune-genotype can provide essential details in tracking and defining the CLL cell of origin.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- B-cells
- CD5
- chronic lymphocytic leukemia (CLL)
- CLL stereotyped BCR
- Ig light chain
- IGHV somatic mutations
- immunoglobulin repertoire
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Bagnara, Davide
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Colombo, Monica
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Reverberi, Danie ...
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Matis, Serena
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Massara, Rosanna
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Cardente, Niccol ...
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show more...
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Ubezio, Gianluca
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Agostini, Vaness ...
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Agnelli, Luca
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Neri, Antonino
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Cardillo, Martin ...
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Vergani, Stefano
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Ghiotto, Fabio
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Mazzarello, Andr ...
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Morabito, Fortun ...
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Cutrona, Giovann ...
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Ferrarini, Manli ...
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Fais, Franco
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Frontiers in Onc ...
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Lund University