Search: onr:"swepub:oai:lup.lub.lu.se:d9d7a82b-dba2-4be9-9b95-60a2a7cba3da" >
Cartilage Oligomeri...
Cartilage Oligomeric Matrix Protein Inhibits Vascular Smooth Muscle Calcification by Interacting With Bone Morphogenetic Protein-2.
-
Du, Yaoyao (author)
-
Wang, Yue (author)
-
Wang, Li (author)
-
show more...
-
Liu, Bo (author)
-
Tian, Qingyun (author)
-
Liu, Chuan-Ju (author)
-
Zhang, Tao (author)
-
Xu, Qingbo (author)
-
Zhu, Yi (author)
-
- Oldberg, Åke (author)
- Lund University,Lunds universitet,Åke Oldbergs forskargrupp,Forskargrupper vid Lunds universitet,Åke Oldberg´s group,Lund University Research Groups
-
Qi, Yongfen (author)
-
Tang, Chaochu (author)
-
Kong, Wei (author)
-
- Wang, Xiangdong (author)
- Lund University,Lunds universitet,Kirurgi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Surgery (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine
-
show less...
-
(creator_code:org_t)
- 2011
- 2011
- English.
-
In: Circulation Research. - 1524-4571. ; 108, s. 79-917
- Related links:
-
http://www.ncbi.nlm....
-
show more...
-
http://dx.doi.org/10...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Rationale: Vascular calcification is a significant contributor to cardiovascular morbidity and mortality. We recently reported that cartilage oligomeric matrix protein (COMP) is pivotal for maintaining the homeostasis of vascular smooth muscle cells (VSMCs). Whether COMP affects the process of vascular calcification is unknown. Objective: We aimed to test whether COMP modulates vascular calcification. Methods and Results: VSMC calcification in vitro was induced by calcifying media containing high inorganic phosphate or calcium. In vivo medial vessel calcification was induced in rats by 5/6 nephrectomy with a high-phosphate diet or by periadventitial application of CaCl(2) to the abdominal aorta. COMP protein level was markedly reduced in both calcified VSMCs and arteries. COMP deficiency remarkably exacerbated VSMC calcification, whereas ectopic expression of COMP greatly reduced calcification. Furthermore, COMP knockdown facilitated osteogenic markers expression by VSMCs even in the absence of calcifying media. By contrast, COMP overexpression significantly inhibited high phosphate- or high calcium-induced VSMC osteochondrogenic transition. Induction of osteogenic marker expression by COMP silencing was reversed by a soluble form of bone morphogenetic protein (BMP)-2 receptor IA, which suggests a BMP-2-dependent mechanism. Our data revealed that COMP bound directly to BMP-2 through the C terminus, inhibited BMP-2 receptor binding, and blocked BMP-2 osteogenic signaling, indicating COMP inhibits osteochondrogenic transition of VSMCs at least partially through inhibiting BMP-2. Conclusions: Our data strongly suggest that COMP is a novel inhibitor of vascular calcification. The imbalance between the effects of COMP and BMP-2 may provide new insights into the pathophysiology of vascular calcification.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Du, Yaoyao
-
Wang, Yue
-
Wang, Li
-
Liu, Bo
-
Tian, Qingyun
-
Liu, Chuan-Ju
-
show more...
-
Zhang, Tao
-
Xu, Qingbo
-
Zhu, Yi
-
Oldberg, Åke
-
Qi, Yongfen
-
Tang, Chaochu
-
Kong, Wei
-
Wang, Xiangdong
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cardiac and Card ...
- Articles in the publication
-
Circulation Rese ...
- By the university
-
Lund University