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- Ding, Wei-Qun (author)
Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways
- Article/chapterEnglish1998
Publisher, publication year, extent ...
- 2002-11-14
- Wiley,1998
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:daf2abd2-2c23-4560-a0bf-93275d780437
- https://lup.lub.lu.se/record/1112630URI
- https://doi.org/10.1046/j.1471-4159.1998.70041722.xDOI
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- Language:English
- Summary in:English
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Notes
- The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C with phorbol ester or by elevating the intracellular Ca2+ concentration with a Ca2+ ionophore. The Ca2+ effect was inhibited by KN-62, suggesting an induction through Ca2+/calmodulin-dependent kinase II. Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Comparison of the inhibitory effects of GF109203X and Go 6976 suggests the involvement of classical protein kinase C isozymes in muscarinic receptor-stimulated expression of fos and jun genes. These results demonstrate that the muscarinic receptor-induced expression of individual fos and jun genes is regulated via different pathways, primarily protein kinase C or Ca2+/calmodulin-dependent kinase II.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
- Muscarinic receptor
- Protein kinase C
- Immediate-early genes
- SH-SY5Y neuroblastoma cells
- Ca2+/calmodulin-dependent kinase II
Added entries (persons, corporate bodies, meetings, titles ...)
- Larsson, ChristerLund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine(Swepub:lu)molm-cla (author)
- Alling, ChristerLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)pneu-cal (author)
- Institutionen för translationell medicinMedicinska fakulteten (creator_code:org_t)
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- In:Journal of Neurochemistry: Wiley70:4, s. 1722-17291471-41590022-3042
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