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Redox sensing by a ...
Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
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Pagels, Martin (author)
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Fuchs, Stephan (author)
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Pane-Farre, Jan (author)
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Kohler, Christian (author)
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Menschner, Leonhard (author)
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Hecker, Michael (author)
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McNamarra, Peter J. (author)
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- Bauer, Mikael (author)
- Lund University,Lunds universitet,Biofysikalisk kemi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biophysical Chemistry,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- von Wachenfeldt, Claes (author)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
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Liebeke, Manuel (author)
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Lalk, Michael (author)
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Sander, Gunnar (author)
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von Eiff, Christof (author)
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Proctor, Richard A. (author)
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Engelmann, Susanne (author)
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(creator_code:org_t)
- 2010-03-30
- 2010
- English.
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In: Molecular Microbiology. - : Wiley. - 1365-2958 .- 0950-382X. ; 76:5, s. 1142-1161
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Abstract
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- P>An alignment of upstream regions of anaerobically induced genes in Staphylococcus aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in Streptomyces coelicolor. Gel shift experiments of selected upstream regions demonstrated that the redox-sensing regulator Rex of S. aureus binds to this inverted repeat. The binding sequence - TTGTGAAW(4)TTCACAA - is highly conserved in S. aureus. Rex binding to this sequence leads to the repression of genes located downstream. The binding activity of Rex is enhanced by NAD+ while NADH, which competes with NAD+ for Rex binding, decreases the activity of Rex. The impact of Rex on global protein synthesis and on the activity of fermentation pathways under aerobic and anaerobic conditions was analysed by using a rex-deficient strain. A direct regulatory effect of Rex on the expression of pathways that lead to anaerobic NAD+ regeneration, such as lactate, formate and ethanol formation, nitrate respiration, and ATP synthesis, is verified. Rex can be considered a central regulator of anaerobic metabolism in S. aureus. Since the activity of lactate dehydrogenase enables S. aureus to resist NO stress and thus the innate immune response, our data suggest that deactivation of Rex is a prerequisite for this phenomenon.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
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- art (subject category)
- ref (subject category)
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Pagels, Martin
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Fuchs, Stephan
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Pane-Farre, Jan
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Kohler, Christia ...
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Menschner, Leonh ...
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Hecker, Michael
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show more...
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McNamarra, Peter ...
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Bauer, Mikael
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von Wachenfeldt, ...
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Liebeke, Manuel
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Lalk, Michael
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Sander, Gunnar
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von Eiff, Christ ...
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Proctor, Richard ...
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Engelmann, Susan ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Physical Chemist ...
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Molecular Microb ...
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Lund University