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17β-estradiol regul...
17β-estradiol regulates the expression of apolipoprotein M through estrogen receptor α-specific binding motif in its promoter
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- Wei, Jiang (author)
- Third Affiliated Hospital of Soochow University
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- Yu, Yang (author)
- Third Affiliated Hospital of Soochow University
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- Luo, Guang-Hua (author)
- Third Affiliated Hospital of Soochow University
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- Feng, Yue hua (author)
- Third Affiliated Hospital of Soochow University
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- Shi, Yuan-ping (author)
- Third Affiliated Hospital of Soochow University
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- Zhang, Jun (author)
- Third Affiliated Hospital of Soochow University
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- Mu, Qin feng (author)
- Third Affiliated Hospital of Soochow University
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- Yu, Miao mei (author)
- Third Affiliated Hospital of Soochow University
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- Pan, Li li (author)
- Third Affiliated Hospital of Soochow University
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- Berggren-Söderlund, Maria (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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- Nilsson-Ehle, Peter (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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- Zhang, Xiao-Ying (author)
- Third Affiliated Hospital of Soochow University
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- Xu, Ning (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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(creator_code:org_t)
- 2017-03-31
- 2017
- English.
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In: Lipids in Health and Disease. - : Springer Science and Business Media LLC. - 1476-511X. ; 16:1, s. 1-8
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Abstract
Subject headings
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- Background: We have previously demonstrated that estrogen could significantly enhance expression of apolipoprotein M (apoM), whereas the molecular basis of its mechanism is not fully elucidated yet. To further investigate the mechanism behind the estrogen induced up-regulation of apoM expression. Results: Our results demonstrated either free 17β-estradiol (E2) or membrane-impermeable bovine serum albumin-conjugated E2 (E2-BSA) could modulate human apoM gene expression via the estrogen receptor alpha (ER-α) pathway in the HepG2 cells. Moreover, experiments with the luciferase activity analysis of truncated apoM promoters could demonstrate that a regulatory region (from-1580 to −1575 bp (−GGTCA-)) upstream of the transcriptional start site of apoM gene was essential for the basal transcriptional activity that regulated by the ER-α. With the applications of an electrophoresis mobility shift assay and a chromatin immunoprecipitation assay, we could successfully identify a specific ER-α binding element in the apoM promoter region. Conculsion: In summary, the present study indicates that 17β-estradiol induced up-regulation of apoM in HepG2 cells is through an ER-α-dependent pathway involving ER-α binding element in the promoter of the apoM gene.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Keyword
- Apolipoprotein M
- Chromatin Immunoprecipitation (ChIP) assay
- Electrophoretic mobility shift assay (EMSA)
- Estrogen receptor alpha
- Estrogen responsive element
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Wei, Jiang
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Yu, Yang
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Luo, Guang-Hua
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Feng, Yue hua
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Shi, Yuan-ping
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Zhang, Jun
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show more...
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Mu, Qin feng
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Yu, Miao mei
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Pan, Li li
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Berggren-Söderlu ...
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Nilsson-Ehle, Pe ...
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Zhang, Xiao-Ying
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Xu, Ning
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Physiology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medicinal Chemis ...
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Lipids in Health ...
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Lund University