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Measurements of the...
Measurements of the binding force between the Helicobacter pylori adhesin BabA and the Lewis b blood group antigen using optical tweezers
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- Björnham, Oscar, 1976- (author)
- Umeå universitet,Institutionen för fysik
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- Fällman, Erik (author)
- Umeå universitet,Institutionen för fysik
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- Axner, Ove (author)
- Umeå universitet,Institutionen för fysik
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- Ohlsson, Jörgen (author)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Ulf (author)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Borén, Thomas (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Schedin, Staffan (author)
- Umeå universitet,Institutionen för tillämpad fysik och elektronik
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(creator_code:org_t)
- Bellingham, WA : SPIE-Intl Soc Optical Eng, 2005
- 2005
- English.
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In: Journal of Biomedical Optics. - Bellingham, WA : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 10:4, s. 044024-044032
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Abstract
Subject headings
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- Helicobacter pylori is a world-wide spread bacterium that causes persistent infections and chronic inflammations that can develop into gastritis and peptic ulcer disease. It expresses several adhesin proteins on its surface that bind to specific receptors in the gastric epithelium. The most well-known adhesin is BabA, which has previously been shown to bind specifically to the fucosylated blood group antigen Lewis b (Leb). The adhesion forces between BabA and the Leb antigen are investigated in this work and assessed by means of optical tweezers. A model system for in situ measurements of the interaction forces between individual bacteria and beads coated with Leb is developed. It is found that the de-adhesion force in this model system, measured with a loading rate of ~100 pN/s, ranges from 20 to 200 pN. The de-adhesion force appears predominantly as multiples of an elementary force, which is determined to 25±1.5 pN and identified as the unbinding force of an individual BabA-Leb binding. It is concluded that adhesion in general is mediated by a small number of bindings (most often 1 to 4) despite that the contact surface between the bacterium and the bead encompassed significantly more binding sites.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioteknisk apparatteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioengineering Equipment (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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