Search: onr:"swepub:oai:lup.lub.lu.se:dfb40dbf-46ae-4ada-9c3d-8da746b0c760" > Characterization of...
Fältnamn | Indikatorer | Metadata |
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000 | 04352naa a2200385 4500 | |
001 | oai:lup.lub.lu.se:dfb40dbf-46ae-4ada-9c3d-8da746b0c760 | |
003 | SwePub | |
008 | 160401s2004 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/1406652 URI |
024 | 7 | a https://doi.org/10.1016/j.jchromb.2004.01.0502 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Plieva, Fatimau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-fpl |
245 | 1 0 | a Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles |
264 | 1 | b Elsevier BV,c 2004 |
520 | a Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel. (C) 2004 Elsevier B.V. All rights reserved. | |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
653 | a Monolithic column | |
653 | a Bioparticles | |
653 | a Cryogel | |
653 | a Supermacroporous polyacrylamide | |
700 | 1 | a Savina, Irinau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-is0 |
700 | 1 | a Deraz, Saharu Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-sd8 |
700 | 1 | a Andersson, Jonatanu Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-ja8 |
700 | 1 | a Galaev, Igoru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-iga |
700 | 1 | a Mattiasson, Bou Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-bma |
710 | 2 | a Bioteknikb Centrum för tillämpade biovetenskaper4 org |
773 | 0 | t Journal of Chromatography. Bd : Elsevier BVg 807:1, s. 129-137q 807:1<129-137x 1873-376Xx 1570-0232 |
856 | 4 | u http://dx.doi.org/10.1016/j.jchromb.2004.01.050y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/140665 |
856 | 4 8 | u https://doi.org/10.1016/j.jchromb.2004.01.050 |
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