Search: onr:"swepub:oai:lup.lub.lu.se:e1b60156-f7a9-462c-a6c9-eff225cd97c0" >
Dihydromyricetin an...
Dihydromyricetin and Salvianolic acid B inhibit alpha-synuclein aggregation and enhance chaperone-mediated autophagy
-
- Wu, Jia-Zhen (author)
- Northeastern University,Sheng Jing Hospital,China Medical University, Shenyang
-
- Ardah, Mustafa (author)
- United Arab Emirates University
-
- Haikal, Caroline (author)
- Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups
-
show more...
-
- Svanbergsson, Alexander (author)
- Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups
-
- Diepenbroek, Meike (author)
- Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups
-
- Vaikath, Nishant N (author)
- Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,Hamad Bin Khalifa University
-
- Li, Wen (author)
- Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups
-
- Wang, Zhan-You (author)
- China Medical University, Shenyang
-
- Outeiro, Tiago F (author)
- University Medical Center Göttingen,Max Planck Institute of Experimental Medicine
-
- El-Agnaf, Omar M (author)
- Hamad Bin Khalifa University
-
- Li, Jia-Yi (author)
- Lund University,Lunds universitet,Neural plasticitet och reparation,Forskargrupper vid Lunds universitet,Neural Plasticity and Repair,Lund University Research Groups,Northeastern University,China Medical University, Shenyang
-
show less...
-
(creator_code:org_t)
- 2019-06-15
- 2019
- English.
-
In: Translational Neurodegeneration. - : Springer Science and Business Media LLC. - 2047-9158. ; 8
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://translationa...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background: Progressive accumulation of α-synuclein is a key step in the pathological development of Parkinson's disease. Impaired protein degradation and increased levels of α-synuclein may trigger a pathological aggregation in vitro and in vivo. The chaperone-mediated autophagy (CMA) pathway is involved in the intracellular degradation processes of α-synuclein. Dysfunction of the CMA pathway impairs α-synuclein degradation and causes cytotoxicity.Results: In the present study, we investigated the effects on the CMA pathway and α-synuclein aggregation using bioactive ingredients (Dihydromyricetin (DHM) and Salvianolic acid B (Sal B)) extracted from natural medicinal plants. In both cell-free and cellular models of α-synuclein aggregation, after administration of DHM and Sal B, we observed significant inhibition of α-synuclein accumulation and aggregation. Cells were co-transfected with a C-terminal modified α-synuclein (SynT) and synphilin-1, and then treated with DHM (10 μM) and Sal B (50 μM) 16 hours after transfection; levels of α-synuclein aggregation decreased significantly (68% for DHM and 75% for Sal B). Concomitantly, we detected increased levels of LAMP-1 (a marker of lysosomal homeostasis) and LAMP-2A (a key marker of CMA). Immunofluorescence analyses showed increased colocalization between LAMP-1 and LAMP-2A with α-synuclein inclusions after treatment with DHM and Sal B. We also found increased levels of LAMP-1 and LAMP-2A both in vitro and in vivo, along with decreased levels of α-synuclein. Moreover, DHM and Sal B treatments exhibited anti-inflammatory activities, preventing astroglia- and microglia-mediated neuroinflammation in BAC-α-syn-GFP transgenic mice.Conclusions: Our data indicate that DHM and Sal B are effective in modulating α-synuclein accumulation and aggregate formation and augmenting activation of CMA, holding potential for the treatment of Parkinson's disease.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Wu, Jia-Zhen
-
Ardah, Mustafa
-
Haikal, Caroline
-
Svanbergsson, Al ...
-
Diepenbroek, Mei ...
-
Vaikath, Nishant ...
-
show more...
-
Li, Wen
-
Wang, Zhan-You
-
Outeiro, Tiago F
-
El-Agnaf, Omar M
-
Li, Jia-Yi
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Neurology
- Articles in the publication
-
Translational Ne ...
- By the university
-
Lund University