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A selected reaction...
A selected reaction monitoring mass spectrometric assessment of biomarker candidates diagnosing large-cell neuroendocrine lung carcinoma by the scaling method using endogenous references
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- Fukuda, Tetsuya (author)
- Biosys Technologies, Inc
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- Nomura, Masaharu (author)
- Tokyo Medical University
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- Kato, Yasufumi (author)
- Tokyo Medical University
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- Tojo, Hiromasa (author)
- Osaka University
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- Fujii, Kiyonaga (author)
- St. Marianna University School of Medicine
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- Nagao, Toshitaka (author)
- Tokyo Medical University
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- Bando, Yasuhiko (author)
- Biosys Technologies, Inc
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- Fehniger, Thomas E. (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Marko-Varga, György (author)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Nakamura, Haruhiko (author)
- St. Marianna University School of Medicine
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- Kato, Harubumi (author)
- Tokyo Medical University,Niizashiki Central General Hospital
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- Nishimura, Toshihide (author)
- Tokyo Medical University
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(creator_code:org_t)
- 2017-04-27
- 2017
- English.
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In: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 12:4
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Abstract
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- Selected reaction monitoring mass spectrometry (SRM-MS) -based semi-quantitation was performed to assess the validity of 46 selected candidate proteins for specifically diagnosing large-cell neuroendocrine lung carcinoma (LCNEC) and differentiating it from other lung cancer subtypes. The scaling method was applied in this study using specific SRM peak areas (AUCs) derived from the endogenous reference protein that normalizes all SRM AUCs obtained for the candidate proteins. In a screening verification study, we found that seven out of the 46 candidate proteins were statistically significant for the LCNEC phenotype, including 4F2hc cell surface antigen heavy chain (4F2hc/CD98) (p-ANOVA ≤ 0.0012), retinal dehydrogenase 1 (p-ANOVA ≤ 0.0029), apolipoprotein A-I (p-ANOVA ≤ 0.0004), β-enolase (p-ANOVA ≤ 0.0043), creatine kinase B-type (p-ANOVA ≤ 0.0070), and galectin- 3-binding protein (p-ANOVA = 0.0080), and phosphatidylethanolamine-binding protein 1 (p-ANOVA ≤ 0.0012). In addition, we also identified candidate proteins specific to the smallcell lung carcinoma (SCLC) subtype. These candidates include brain acid soluble protein 1 (p-ANOVA < 0.0001) and γ-enolase (p-ANOVA ≤ 0.0013). This new relative quantitationbased approach utilizing the scaling method can be applied to assess hundreds of protein candidates obtained from discovery proteomic studies as a first step of the verification phase in biomarker development processes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
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- By the author/editor
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Fukuda, Tetsuya
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Nomura, Masaharu
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Kato, Yasufumi
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Tojo, Hiromasa
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Fujii, Kiyonaga
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Nagao, Toshitaka
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show more...
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Bando, Yasuhiko
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Fehniger, Thomas ...
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Marko-Varga, Gyö ...
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Nakamura, Haruhi ...
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Kato, Harubumi
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Nishimura, Toshi ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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PLoS ONE
- By the university
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Lund University