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Formation of amyloi...
Formation of amyloid-like fibrils upon limited proteolysis of bovine alpha-lactalbumin
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Otte, J (author)
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Ipsen, R (author)
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Bauer, R (author)
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Bjerrum, MJ (author)
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- Waninge, Rianne (author)
- Lund University,Lunds universitet,Livsmedelsteknik,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Food Technology, Engineering and Nutrition,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
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In: International Dairy Journal. - : Elsevier BV. - 0958-6946. ; 15:3, s. 219-229
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Bovine alpha-lactalbumin (alpha-LA) (10 g L-1) was incubated with a protease from Bacillus licheniformis at pH 7.5 and 50 degreesC. The reaction was biphasic consisting of an initial hydrolysis of intact alpha-LA and formation of dimers from large hydrolysis products within 60 min followed by aggregation of dimers into aggregates of 500 kDa. The aggregates consisted primarily of fibrillar strands with a diameter of 5 nm. Formation of these strands was accompanied by a change in secondary structure towards higher beta-sheet content and strong binding of thioflavin, features shared with amyloidal fibrils. The main components in these fibrils were fragments of 8.8 and 9.8 kDa shown to occur in a monomer-dimer equilibrium. These fragments were identified and a molecular mechanism involving side-by-side assembly of dimers of these fragments into fibrils is proposed.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Annan teknik -- Livsmedelsteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies -- Food Engineering (hsv//eng)
Keyword
- amyloid fibrils
- assembly
- fragments
- alpha-lactalbumin
- proteolysis
Publication and Content Type
- art (subject category)
- ref (subject category)
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