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Epitope mapping of ...
Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells
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- Persson, Nina (author)
- University of Copenhagen
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- Stuhr-Hansen, Nicolai (author)
- University of Copenhagen
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- Risinger, Christian (author)
- University of Copenhagen
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- Mereiter, Stefan (author)
- University of Porto
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- Polónia, António (author)
- University of Porto
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- Polom, Karol (author)
- University of Siena
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- Kovács, András (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,University of Copenhagen
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- Roviello, Franco (author)
- University of Siena
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- Reis, Celso A. (author)
- University of Porto
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- Welinder, Charlotte (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,CEBMMS PI,Forskargrupper vid Lunds universitet,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups
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- Danielsson, Lena (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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- Jansson, Bo (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Blixt, Ola (author)
- University of Copenhagen
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(creator_code:org_t)
- 2017-05-04
- 2017
- English 11 s.
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In: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 27:7, s. 635-645
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Here, we introduce a novel scFv antibody, G2-D11, specific for two adjacent Tn-antigens (GalNAc- Ser/Thr) binding equally to three dimeric forms of the epitope, Ser-Thr, Thr-Thr and Thr-Ser. Compared to other anti-Tn reagents, the binding of G2-D11 is minimally influenced by the peptide structure, which indicates a high degree of carbohydrate epitope dominance and a low influence from the protein backbone. With a high affinity (KDapp = 1.3 × 10-8 M) and no cross-reactivity to either sialyl-Tn epitope or blood group A antigens, scFv G2-D11 is an excellent candidate for a well-defined anti-Tn-antigen reagent. Detailed immunohistochemical evaluation of tissue sections from a cohort of 80 patients with gastric carcinoma showed in all cases positive tumor cells. The observed staining was localized to the cytoplasm and in some cases to the membrane, whereas the surrounding tissue was completely negative demonstrating the usefulness of the novel Tn-antigen binding antibody.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- gastric cancer
- glycosylation
- N-acetylgalactosamine (GalNAc)
- scFv
- solid-phase peptide synthesis
- Tn-antigen
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Persson, Nina
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Stuhr-Hansen, Ni ...
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Risinger, Christ ...
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Mereiter, Stefan
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Polónia, António
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Polom, Karol
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show more...
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Kovács, András
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Roviello, Franco
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Reis, Celso A.
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Welinder, Charlo ...
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Danielsson, Lena
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Jansson, Bo
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Blixt, Ola
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Glycobiology
- By the university
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Lund University