Neurogenesis in the adult spinal cord in an experimental model of multiple sclerosis

Danilov, AlexandreLund University,Lunds universitet,Neurogenes och Cellterapi,Forskargrupper vid Lunds universitet,Neurogenesis and cell therapy,Lund University Research Groups (author)

Multiple sclerosis is an inflammatory disease of the central nervous system characterized by inflammation, demyelination, axonal degeneration and accumulation of neurological disability. Previously, we demonstrated that stem cells constitute a possible endogenous source for remyelination. We now addressed the question of whether neurogenesis can occur in neuroinflammatory lesions. We demonstrated that, in experimental autoimmune encephalomyelitis, induced in rats 1,1'-dioctadecyl-6,6'-di(4sulphopentyl)-3,3,3',3'tetramethylindocarbocyani n(DiI)-labelled ependymal cells not only proliferated but descendants migrated to the area of neuroinflammation and differentiated into cells expressing the neuronal markers beta-III-tubulin and NeuN. Furthermore, these cells were immunoreactive for bromodeoxyuridine and PCNA, markers for cells undergoing cell proliferation. Using the whole-cell patch-clamp technique on freshly isolated 1, DiI-labelled cells from spinal cord lesions we demonstrated the ability of these cells to fire overshooting action potentials similar to those of immature neurones. We thus provide the first evidence for the initiation of neurogenesis in neuroinflammatory lesions in the adult spinal cord.

Covacu, RuxandraKarolinska Institutet (author)
Moe, Morten C (author)
Langmoen, Iver A (author)
Johansson, Clas BKarolinska Institutet (author)
Olsson, TomasKarolinska Institutet (author)
Brundin, LouKarolinska Institutet (author)
Neurogenes och CellterapiForskargrupper vid Lunds universitet (creator_code:org_t)

By the author/editor: Danilov, Alexand ...; Covacu, Ruxandra; Moe, Morten C; Langmoen, Iver A; Johansson, Clas ...; Olsson, Tomas; show more...; Brundin, Lou; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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