Search: onr:"swepub:oai:lup.lub.lu.se:e7f8b45c-8d3c-4007-9d65-81bfe13cb134" >
Implementation of F...
Implementation of Formalin-Fixed, Paraffin-Embedded Cell Line Pellets as High-Quality Process Controls in Quality Assessment Programs for KRAS Mutation Analysis
-
Dijkstra, Jeroen R. (author)
-
Opdam, Frank J. M. (author)
-
Boonyaratanakornkit, Jerry (author)
-
show more...
-
Schoenbrunner, E. Ralf (author)
-
Shahbazian, Mona (author)
-
- Edsjö, Anders (author)
- Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine
-
Hoefler, Gerald (author)
-
Jung, Andreas (author)
-
Kotsinas, Athanassios (author)
-
Gorgoulis, Vassilis G. (author)
-
Lopez-Rios, Fernando (author)
-
de Stricker, Karin (author)
-
Rouleau, Etienne (author)
-
Biesmans, Bart (author)
-
van Krieken, J. Han J. M. (author)
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
-
In: The Journal Of Molecular Diagnostics. - : Elsevier BV. - 1525-1578. ; 14:3, s. 187-191
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
http://www.jmdjourna...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- In recent years, the mutational status of the KRAS oncogene has become incorporated into standard medical care as a predictive marker for therapeutic decisions related to patients with metastasized colorectal cancer. This is necessary, because these patients benefit from epidermal growth factor receptor (EGFR)-targeted therapy with increased progression-free survival only if the tumor does not carry a mutation in KRAS. Many different analytical platforms, both those commercially available and those developed in house, have been used within pathology laboratories to assess KRAS mutational status. For a testing laboratory to become accredited to perform such tests, it is essential that they perform reliability testing, but it has not previously been possible to perform this kind of testing on the complete workflow on a large scale without compromising reproducibility or the mimicry of the control sample. We assessed a novel synthetic control for formalin-fixed, paraffin-embedded (FFPE) tumor samples in a blind study conducted within nine laboratories across Europe. We show that FFPE material can, at least in part, mimic clinical samples and we demonstrate this control to be a valuable tool in the assessment of platforms used In testing for KRAS mutational status. (J Mal Diagn 2012; 14:187-191; DOI: 10.1016/j.jmoldx.2012.01.002).
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Dijkstra, Jeroen ...
-
Opdam, Frank J. ...
-
Boonyaratanakorn ...
-
Schoenbrunner, E ...
-
Shahbazian, Mona
-
Edsjö, Anders
-
show more...
-
Hoefler, Gerald
-
Jung, Andreas
-
Kotsinas, Athana ...
-
Gorgoulis, Vassi ...
-
Lopez-Rios, Fern ...
-
de Stricker, Kar ...
-
Rouleau, Etienne
-
Biesmans, Bart
-
van Krieken, J. ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
- Articles in the publication
-
The Journal Of M ...
- By the university
-
Lund University