Search: onr:"swepub:oai:lup.lub.lu.se:e82c0dda-a889-40d5-be13-83e2078bd61e" >
Aberrant recombinat...
-
Björkman, AndreaKarolinska Institutet
(author)
Aberrant recombination and repair during immunoglobulin class switching in BRCA1-deficient human B cells.
- Article/chapterEnglish2015
Publisher, publication year, extent ...
-
2015-02-02
-
Proceedings of the National Academy of Sciences,2015
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:lup.lub.lu.se:e82c0dda-a889-40d5-be13-83e2078bd61e
-
https://lup.lub.lu.se/record/5145596URI
-
https://doi.org/10.1073/pnas.1418947112DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:130694802URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:art swepub-publicationtype
-
Subject category:ref swepub-contenttype
Notes
-
Breast cancer type 1 susceptibility protein (BRCA1) has a multitude of functions that contribute to genome integrity and tumor suppression. Its participation in the repair of DNA double-strand breaks (DSBs) during homologous recombination (HR) is well recognized, whereas its involvement in the second major DSB repair pathway, nonhomologous end-joining (NHEJ), remains controversial. Here we have studied the role of BRCA1 in the repair of DSBs in switch (S) regions during immunoglobulin class switch recombination, a physiological, deletion/recombination process that relies on the classical NHEJ machinery. A shift to the use of microhomology-based, alternative end-joining (A-EJ) and increased frequencies of intra-S region deletions as well as insertions of inverted S sequences were observed at the recombination junctions amplified from BRCA1-deficient human B cells. Furthermore, increased use of long microhomologies was found at recombination junctions derived from E3 ubiquitin-protein ligase RNF168-deficient, Fanconi anemia group J protein (FACJ, BRIP1)-deficient, or DNA endonuclease RBBP8 (CtIP)-compromised cells, whereas an increased frequency of S-region inversions was observed in breast cancer type 2 susceptibility protein (BRCA2)-deficient cells. Thus, BRCA1, together with its interaction partners, seems to play an important role in repairing DSBs generated during class switch recombination by promoting the classical NHEJ pathway. This may not only provide a general mechanism underlying BRCA1's function in maintaining genome stability and tumor suppression but may also point to a previously unrecognized role of BRCA1 in B-cell lymphomagenesis.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Qvist, Per
(author)
-
Du, LikunKarolinska Institutet
(author)
-
Bartish, MargaritaKarolinska Institutet
(author)
-
Zaravinos, Apostolos
(author)
-
Georgiou, KonstantinosKarolinska Institutet
(author)
-
Børglum, Anders D
(author)
-
Gatti, Richard A
(author)
-
Törngren, ThereseLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)onk-tsa
(author)
-
Pan-Hammarström, QiangKarolinska Institutet
(author)
-
Karolinska InstitutetBröstcancer-genetik
(creator_code:org_t)
Related titles
-
In:Proceedings of the National Academy of Sciences: Proceedings of the National Academy of Sciences112:7, s. 2157-21621091-64900027-8424
Internet link
Find in a library
To the university's database
- By the author/editor
-
Björkman, Andrea
-
Qvist, Per
-
Du, Likun
-
Bartish, Margari ...
-
Zaravinos, Apost ...
-
Georgiou, Konsta ...
-
show more...
-
Børglum, Anders ...
-
Gatti, Richard A
-
Törngren, Theres ...
-
Pan-Hammarström, ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
- Articles in the publication
-
Proceedings of t ...
- By the university
-
Lund University
-
Karolinska Institutet