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Evaluation of seven...
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Ryding, UlfLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
(author)
Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia
- Article/chapterEnglish2002
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LIBRIS-ID:oai:lup.lub.lu.se:e9d30f1b-97a3-45cc-a1cd-f39ff713ba97
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https://lup.lub.lu.se/record/343920URI
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https://doi.org/10.1016/S0732-8893(01)00311-XDOI
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Language:English
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Summary in:English
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Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid, a-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia. (C) 2002 Elsevier Science Inc. All rights reserved.
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Espersen, F
(author)
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Soderquist, B
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Christensson, B
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InfektionsmedicinSektion III
(creator_code:org_t)
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In:Diagnostic Microbiology and Infectious Disease42:1, s. 9-151879-0070
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