onr:"swepub:oai:lup.lub.lu.se:ec033e97-d731-4ede-bb60-7e348154702e"
Search: onr:"swepub:oai:lup.lub.lu.se:ec033e97-d731-4ede-bb60-7e348154702e" > Longitudinal epitop...
- 1 of 1
- Previous record
- Next record
- To hitlist
Longitudinal epitope analysis of insulin-binding antibodies in type 1 diabetes
- Hall, T. R. (author)
- Thomas, J. W. (author)
- Padoa, C. J. (author)
- show more...
- Hampe, C. S. (author)
- show less...
- (creator_code:org_t)
- 2006-08-16
- 2006
- English.
- In: Clinical and Experimental Immunology. - : Oxford University Press (OUP). - 0009-9104 .- 1365-2249. ; 146:1, s. 41531-41531
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Autoantibodies to insulin (IAA) are one of the first markers of the autoimmune process leading to type 1 diabetes (T1D). While other autoantibodies in T1D have been studied extensively, relatively little is known about IAA and their binding specificities, especially after insulin treatment is initiated. We hypothesize that insulin antibodies (IA) that develop upon initiation of insulin treatment differ in their epitope specificities from IAA. We analysed insulin antibody binding specificities in longitudinal samples of T1D patients (n = 49). Samples were taken at clinical diagnosis of disease and after insulin treatment was initiated. The epitope specificities were analysed using recombinant Fab (rFab) derived from insulin-specific monoclonal antibodies AE9D6 and CG7C7. Binding of radiolabelled insulin by samples taken at onset of the disease was significantly reduced in the presence of rFab CG7C7 and AE9D6. rFab AE9D6 competed sera binding to insulin significantly better than rFab CG7C7 (P = 0.02). Binding to the AE9D6-defined epitope in the initial sample was correlated inversely with age at onset (P = 0.005). The binding to the AE9D6-defined epitope increased significantly (P < 0.0001) after 3 months of insulin treatment. Binding to the CG7C7-defined epitope did not change during the analysed period of 12 months. We conclude that epitopes recognized by insulin binding antibodies can be identified using monoclonal insulin-specific rFab as competitors. Using this approach we observed that insulin treatment is accompanied by a change in epitope specificities in the emerging IA.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- type 1 diabetes
- Fab
- recombinant
- radioligand binding assay
- epitopes
- insulin autoantibody
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
- Clinical and Experimental Immunology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Hall, T. R.
- Thomas, J. W.
- Padoa, C. J.
- Törn, Carina
- Landin-Olsson, M ...
- Ortqvist, E
- show more...
- Hampe, C. S.
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Immunology in th ...
- Articles in the publication
- Clinical and Exp ...
- By the university
- Lund University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
