SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:lup.lub.lu.se:ecb73398-ed17-45da-9b8f-9acdfb8dab85"
 

Search: onr:"swepub:oai:lup.lub.lu.se:ecb73398-ed17-45da-9b8f-9acdfb8dab85" > Small Extracellular...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004876naa a2200541 4500
001oai:lup.lub.lu.se:ecb73398-ed17-45da-9b8f-9acdfb8dab85
003SwePub
008231204s2023 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/ecb73398-ed17-45da-9b8f-9acdfb8dab852 URI
0247 a https://doi.org/10.1016/j.bbrc.2023.09.0222 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Suzuki, Hironoriu National Cancer Center Research Institute, Japan,Nagoya University4 aut
2451 0a Small Extracellular Vesicles from adipose-derived stem cells suppress cell proliferation by delivering the let-7 family of microRNAs in ovarian cancer
264 1c 2023
300 a 9 s.
520 a Introduction: Ovarian cancer is the leading cause of death among women with gynecological cancer, and novel treatment options are urgently needed. Extracellular vesicles (EVs), including exosomes, may be one of the most promising therapeutic tools for various diseases. In this study, we aimed to investigate the therapeutic effects of adipose-derived stem cell-derived EVs (ADSC-EVs) on ovarian cancer cell lines. Materials and methods: ADSCs and the ovarian cancer cell lines SKOV3 and OV90 were used for analysis. ADSC-EVs were isolated through ultracentrifugation and validated using a cryotransmission electron microscope, nanoparticle tracking analysis, and western blotting. Then, the effect of ADSC-EVs on ovarian cancer cells was investigated using IncuCyte and microRNA sequencing. Moreover, the potential functions of miRNAs were evaluated by gain-of function analysis and in silico analysis. Results: ADSC-EVs suppressed SKOV3 and OV90 cell proliferation. In particular, small EVs (sEVs) from ADSCs exhibited a stronger antitumor effect than ADSC-medium/large EVs (m/lEVs). Comparison of the miRNA profiles between ADSC-sEVs and ADSC-m/lEVs, along with downstream pathway analysis, suggested the involvement of the let-7 family. Overexpression of hsa-let-7b-5p and hsa-let-7e-5p significantly suppressed the proliferation of SKOV3 cells. In silico analysis revealed that four potential target genes of hsa-let-7b-5p and hsa-let-7e-5p were significantly associated with the prognoses of the patients. Conclusion: ADSC-sEVs had a stronger antitumor effect than ADSC-m/lEVs. Hsa-let-7b-5p and hsa-let-7e-5p, which are highly abundant in ADSC-sEVs, suppressed cell proliferation. These findings may open up new possibilities for therapeutic approaches using ADSC-sEVs.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
653 a hsa-let-7b-5p
653 a hsa-let-7e-5p
653 a Medium/large extracellular vesicles
653 a Ovarian cancer
653 a Small extracellular vesicles
7001 a Yokoi, Akirau Nagoya University4 aut
7001 a Uno, Kanameu Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancercellers evolution,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine,Pathways of cancer cell evolution,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Nagoya University4 aut0 (Swepub:lu)ka6202un
7001 a Yoshida, Kosukeu National Cancer Center Research Institute, Japan,Nagoya University4 aut
7001 a Kitagawa, Masamiu Nagoya University4 aut
7001 a Asano-Inami, Eriu Nagoya University4 aut
7001 a Matsuo, Seikou Nagoya University4 aut
7001 a Nagao, Yukariu Nagoya University4 aut
7001 a Suzuki, Kazuhirou Nagoya University4 aut
7001 a Nakamura, Kaeu Nagoya University4 aut
7001 a Yoshihara, Masatou Nagoya University4 aut
7001 a Tamauchi, Satoshiu Nagoya University4 aut
7001 a Shimizu, Yusukeu Nagoya University4 aut
7001 a Ikeda, Yoshikiu Nagoya University4 aut
7001 a Yoshikawa, Nobuhisau Nagoya University4 aut
7001 a Kajiyama, Hiroakiu Nagoya University4 aut
7001 a Yamamoto, Yusukeu National Cancer Center Research Institute, Japan4 aut
7102 a National Cancer Center Research Institute, Japanb Nagoya University4 org
7730 t Biochemical and Biophysical Research Communicationsg 680, s. 211-219q 680<211-219x 0006-291X
8564 u http://dx.doi.org/10.1016/j.bbrc.2023.09.022y FULLTEXT
8564 8u https://lup.lub.lu.se/record/ecb73398-ed17-45da-9b8f-9acdfb8dab85
8564 8u https://doi.org/10.1016/j.bbrc.2023.09.022

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view