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Neuroprotection in ...
Neuroprotection in the rat Parkinson model by intrastriatal GDNF gene transfer using a lentiviral vector.
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- Georgievska, Biljana (author)
- Lund University,Lunds universitet,Neurobiologi,Forskargrupper vid Lunds universitet,Neurobiology,Lund University Research Groups
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- Kirik, Deniz (author)
- Lund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine
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Rosenblad, Carl (author)
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- Lundberg, Cecilia (author)
- Lund University,Lunds universitet,Wallenberg Neurocentrum, Lund,Medicinska fakulteten,Wallenberg Neuroscience Centre, Lund,Faculty of Medicine
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- Björklund, Anders (author)
- Lund University,Lunds universitet,Neurobiologi,Forskargrupper vid Lunds universitet,Neurobiology,Lund University Research Groups
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(creator_code:org_t)
- 2002
- 2002
- English.
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In: NeuroReport. - 1473-558X. ; 13:1, s. 75-82
- Related links:
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http://www.ncbi.nlm....
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Abstract
Subject headings
Close
- We used a recombinant lentiviral vector (rLV) for gene delivery of GDNF to the striatum, and assessed its neuroprotective effects in the intrastriatal 6-hydroxydopamine (6-OHDA) lesion model.The level of GDNF expression obtained with the rLV-GDNF vector was dose-related and ranged between 0.9-9.3 ng/mg tissue in the transduced striatum, as determined by ELISA, and 0.2-3.0 ng/mg tissue were detected in the ipsilateral substantia nigra (SN), due to anterograde transport of the GDNF protein. GDNF expression was apparent at 4 days and maintained for > 8 months after injection. Striatal delivery of rLV-GDNF efficiently protected the nigral dopamine (DA) neurons and their projection, against the 6-OHDA lesion (65-77% of intact side). Sprouting of the lesioned axons was observed along the nigrostriatal pathway, precisely corresponding to the areas containing anterogradely transported GDNF.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Lentivirus : genetics
- Luminescent Proteins : pharmacokinetics
- Motor Activity : drug effects
- Nerve Tissue Proteins : genetics
- Nerve Tissue Proteins : pharmacokinetics
- Nerve Tissue Proteins : therapeutic use
- Neurons : drug effects
- Neurons : metabolism
- Neuroprotective Agents : therapeutic use
- Oxidopamine : pharmacology
- Parkinson Disease : pathology
- Parkinson Disease : physiopathology
- Parkinson Disease : therapy
- Rats
- Sprague-Dawley
- Substantia Nigra : drug effects
- Substantia Nigra : pathology
- Support
- Non-U.S. Gov't
- Tyrosine 3-Monooxygenase : metabolism
- Indicators and Reagents : pharmacokinetics
- Genetic Vectors
- Gene Transfer Techniques
- Female
- Dopamine : metabolism
- Corpus Striatum : pathology
- Corpus Striatum : drug effects
- Animal
Publication and Content Type
- art (subject category)
- ref (subject category)
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