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A novel strategy to...
A novel strategy to protect against influenza-induced pneumococcal disease without interfering with commensal colonization
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- Greene, Christopher J. (author)
- The State University of New York
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- Marks, Laura R. (author)
- The State University of New York
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- Hu, John C. (author)
- The State University of New York
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- Reddinger, Ryan (author)
- The State University of New York
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- Mandell, Lorrie (author)
- The State University of New York
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- Roche-Hakansson, Hazeline (author)
- The State University of New York
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- King-Lyons, Natalie D. (author)
- The State University of New York
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- Connell, Terry D. (author)
- The State University of New York
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- Håkansson, Anders P (author)
- Lund University,Lunds universitet,Experimentell infektionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Experimental Infection Medicine, Malmö,Lund University Research Groups,The State University of New York
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(creator_code:org_t)
- 2016
- 2016
- English.
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In: Infection and Immunity. - 1098-5522. ; 84:6, s. 1693-1703
- Related links:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Streptococcus pneumoniae commonly inhabits the nasopharynx as a member of the commensal biofilm. Infection with respiratory viruses, such as influenza A virus, induces commensal S. pneumoniae to disseminate beyond the nasopharynx and to elicit severe infections of the middle ears, lungs, and blood that are associated with high rates of morbidity and mortality. Current preventive strategies, including the polysaccharide conjugate vaccines aim to eliminate asymptomatic carriage with vaccine-type pneumococci. However, this has resulted in serotype-replacement with, so-far, less fit pneumococcal strains, which has changed the nasopharyngeal flora, opening the niche for entry of other virulent pathogens (e.g., Streptococcus pyogenes, Staphylococcus aureus, and potentially Haemophilus influenzae). The long-term effects of these changes are unknown. Here, we present an attractive, alternative preventive approach where we subvert virally-induced pneumococcal disease without interfering with commensal colonization, thus specifically targeting disease-causing organisms. In that regard, pneumococcal surface protein A (PspA), a major surface protein of pneumococci, is a promising vaccine target. Intradermal (i.d.) immunization of mice with recombinant PspA in combination with LT-IIb(T13I), a novel i.d. adjuvant of the type II heat-labile enterotoxin family, elicited strong systemic PspA-specific IgG responses without inducing mucosal anti-PspA IgA responses. This response protected mice from otitis media, pneumonia, and septicemia and averted the cytokine storm associated with septic infection but had no effect on asymptomatic colonization. Our results firmly demonstrated that this immunization strategy against virally-induced, pneumococcal disease can be conferred without disturbing the desirable pre-existing commensal colonization of the nasopharynx.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- pneumococcal disease
Publication and Content Type
- art (subject category)
- ref (subject category)
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