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Fate Distribution a...
Fate Distribution and Regulatory Role of Human Mesenchymal Stromal Cells in Engineered Hematopoietic Bone Organs
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- Bourgine, Paul E. (author)
- Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Molecular Skeletal Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine,University of Basel,University Hospital Basel,ETH Zürich
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- Fritsch, Kristin (author)
- University Hospital of Zurich,University of Zurich
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- Pigeot, Sebastien (author)
- University of Basel,University Hospital Basel
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- Takizawa, Hitoshi (author)
- Kumamoto University
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- Kunz, Leo (author)
- ETH Zürich
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- Kokkaliaris, Konstantinos D. (author)
- ETH Zürich
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- Coutu, Daniel L. (author)
- ETH Zürich
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- Manz, Markus G. (author)
- University Hospital Basel,University of Basel
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- Martin, Ivan (author)
- University Hospital Basel,University of Basel
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- Schroeder, Timm (author)
- ETH Zürich
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English 10 s.
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In: iScience. - : Elsevier BV. - 2589-0042. ; 19, s. 504-513
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Abstract
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- The generation of humanized ectopic ossicles (hOss) in mice has been proposed as an advanced translational and fundamental model to study the human hematopoietic system. The approach relies on the presence of human bone marrow-derived mesenchymal stromal cells (hMSCs) supporting the engraftment of transplanted human hematopoietic stem and progenitor cells (HSPCs). However, the functional distribution of hMSCs within the humanized microenvironment remains to be investigated. Here, we combined genetic tools and quantitative confocal microscopy to engineer and subsequently analyze hMSCs′ fate and distribution in hOss. Implanted hMSCs reconstituted a humanized environment including osteocytes, osteoblasts, adipocytes, and stromal cells associated with vessels. By imaging full hOss, we identified rare physical interactions between hMSCs and human CD45+/CD34+/CD90+ cells, supporting a functional contact-triggered regulatory role of hMSCs. Our study highlights the importance of compiling quantitative information from humanized organs, to decode the interactions between the hematopoietic and the stromal compartments. Biological Sciences; Stem Cells Research; Tissue Engineering
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Biological Sciences
- Stem Cells Research
- Tissue Engineering
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Bourgine, Paul E ...
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Fritsch, Kristin
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Pigeot, Sebastie ...
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Takizawa, Hitosh ...
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Kunz, Leo
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Kokkaliaris, Kon ...
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show more...
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Coutu, Daniel L.
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Manz, Markus G.
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Martin, Ivan
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Schroeder, Timm
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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iScience
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Lund University