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Proteomic Profiling...
Proteomic Profiling of Diffuse Large B-Cell Lymphomas
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- Kwiecińska, Anna (author)
- Karolinska Institutet,Karolinska Institute,Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Porwit, Anna (author)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Souchelnytskyi, Nazariy (author)
- Oranta Cancer Diagnostics AB
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- Kaufeldt, Ann (author)
- Karolinska University Hospital
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- Larsson, Catharina (author)
- Karolinska Institutet,Karolinska Institute
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- Bajalica-Lagercrantz, Svetlana (author)
- Karolinska Institutet,Karolinska Institute
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- Souchelnytskyi, Serhiy (author)
- Oranta Cancer Diagnostics AB,Qatar University
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(creator_code:org_t)
- 2018-04-04
- 2018
- English.
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In: Pathobiology. - : S. Karger AG. - 1015-2008 .- 1423-0291. ; 85:4, s. 211-219
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Abstract
Subject headings
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- Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- BiP/Grp78
- Cyclin B2
- De novo diffuse large B-cell lymphomas
- Diffuse large B-cell lymphoma
- Germinal center type
- Hsp90
- Non-germinal center type
- Proteomics
- Transformed lymphoma
Publication and Content Type
- art (subject category)
- ref (subject category)
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