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- Paulsson, Kajsa MLund University,Lunds universitet,Antigen presentation,Forskargrupper vid Lunds universitet,Antigen Presentation,Lund University Research Groups (author)
Assembly of tapasin-associated MHC class I in the absence of the transporter associated with antigen processing (TAP)
- Article/chapterEnglish2001
Publisher, publication year, extent ...
- 2001-02
- Oxford University Press (OUP),2001
- 7 s.
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- LIBRIS-ID:oai:lup.lub.lu.se:f66fe82b-15d5-4f5b-963e-718069cb47dc
- https://lup.lub.lu.se/record/f66fe82b-15d5-4f5b-963e-718069cb47dcURI
- https://doi.org/10.1093/intimm/13.1.23DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:1934708URI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- The assembly of MHC class I molecules is regulated by a multi-protein complex in the endoplasmic reticules (ER) termed the loading complex. Tapasin is suggested to be one of the molecules forming this complex on the basis of its interaction with both the transporter associated with antigen processing (TAP) and MHC class I molecules. To address whether TAP is indispensable for the processing of the assembly of tapasin-associated MHC class I molecules, we studied the association of MHC class I molecules with tapasin, the assembly of tapasin-associated MHC class I with peptides and the peptide-mediated dissociation of MHC class I from tapasin in TAP-mutant T2 cells. In the absence of TAP, MHC class I heavy chain and beta(2)-microglobulin dimers were found to be properly associated with tapasin. The stable MHC class I dimer was required for its association with tapasin in the ER. In the absence of TAP, tapasin retained MHC class I molecules much longer in the ER than in the presence of TAP. This low off-rate of MHC class I from tapasin was due to the absence of high-affinity peptides in the ER of TAP-mutant cells but not to the absence of TAP per se. The introduction of peptides into permeabilized microsomes of TAP-mutant cells led to effective loading of the peptides onto tapasin-associated MHC class I and to the subsequent dissociation of MHC class I from tapasin. These results demonstrate that regulation of the assembly of tapasin-associated MHC class I is independent of the interaction of tapasin with TAP, but is dependent upon the peptides transported by TAP.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- ATP-Binding Cassette Transporters
- Adenosine Triphosphate
- Antigen Presentation
- Antiporters
- Cell Line
- HLA Antigens
- Histocompatibility Antigens Class I
- Humans
- Immunoglobulins
- Membrane Transport Proteins
- Mutation
- Peptides
- Protein Binding
- Journal Article
- Research Support, Non-U.S. Gov't
- tapasin
- MHC-I
- antigen presentation
Added entries (persons, corporate bodies, meetings, titles ...)
- Anderson, P OLund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups(Swepub:lu)wbla-pan (author)
- Chen, ShangwuLund University (author)
- Sjögren, H OLund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)wbl-hos (author)
- Ljunggren, H-GKarolinska Institutet (author)
- Wang, PQueen Mary University (author)
- Li, SBrunel University London(Swepub:lu)wbl-sll (author)
- Antigen presentationForskargrupper vid Lunds universitet (creator_code:org_t)
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- In:International Immunology: Oxford University Press (OUP)13:1, s. 9-230953-81781460-2377
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