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Modulation of invas...
Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C
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Coulibaly, S (author)
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Schwihla, H (author)
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- Abrahamson, Magnus (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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Albini, A (author)
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Cerni, C (author)
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Clark, JL (author)
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Ng, KM (author)
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Katunuma, N (author)
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Schlappack, O (author)
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Glossl, J (author)
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Mach, L (author)
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(creator_code:org_t)
- 1999
- 1999
- English.
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In: International Journal of Cancer. - 0020-7136. ; 83:4, s. 526-531
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http://dx.doi.org/10...
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Abstract
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- Murine SCC-VII squamous carcinoma cells have the capacity to penetrate reconstituted basement membranes (Matrigel) in vitro. The invasion of Matrigel layers by SCC-VII cells was significantly reduced by E-64, a specific inhibitor of lysosomal cysteine proteinases. The cathepsin-B-selective E-64 derivative, CA-074, inhibited penetration of Matrigel by SCC-VII cells to the same extent, indicating a major role for this particular lysosomal enzyme in extracellular-matrix degradation during squamous-carcinoma-cell invasion. SCC-VII cells were stably transfected with a cDNA encoding human procathepsin B, in an attempt to modulate the invasive properties of the cell line. The transfected cells expressed the heterologous gene, secreted increased amounts of procathepsin B and displayed enhanced invasive potential. In vivo, the activity of cathepsin B is strictly regulated by endogenous inhibitors. SCC-VII cells were therefore also stably transfected with a cDNA encoding human cystatin C, the most potent cysteine-proteinase inhibitor in mammalian tissues. The expression of this transgene resulted in the production of active recombinant cystatin C and a pronounced reduction in Matrigel invasion. These studies demonstrate that the invasive properties of squamous-cell carcinomas can be changed by modulation of the balance between cathepsin B and its endogenous inhibitors, and provide further evidence for the involvement of this lysosomal cysteine proteinase in tumour invasion and metastasis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
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- art (subject category)
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- By the author/editor
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Coulibaly, S
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Schwihla, H
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Abrahamson, Magn ...
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Albini, A
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Cerni, C
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Clark, JL
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show more...
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Ng, KM
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Katunuma, N
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Schlappack, O
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Glossl, J
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Mach, L
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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Lund University