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Lipopolysaccharide ...
Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.
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Arciszewski, Marcin (author)
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- Pierzynowski, Stefan (author)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science
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- Ekblad, Eva (author)
- Lund University,Lunds universitet,Neurogastroenterologi,Forskargrupper vid Lunds universitet,Neurogastroenterology,Lund University Research Groups
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2005
- 2005
- English.
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In: Digestive Diseases and Sciences. - : Springer Science and Business Media LLC. - 1573-2568 .- 0163-2116. ; 50:9, s. 1661-1668
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Abstract
Subject headings
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- Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Keyword
- Myenteric Plexus: cytology
- Animals
- Cell Culture Techniques
- Cell Death
- Cell Survival
- Female
- Immunohistochemistry
- Inflammation
- Intestinal Diseases: etiology
- Intestinal Diseases: immunology
- Intestinal Diseases: microbiology
- Lipopolysaccharides: pharmacology
- Male
- Myenteric Plexus: pathology
- Neurons: drug effects
- Neurons: physiology
- Neurotransmitters: biosynthesis
- Research Support
- Non-U.S. Gov't
- Swine
Publication and Content Type
- art (subject category)
- ref (subject category)
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