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Sample preparation ...
Sample preparation approach influences pam50 risk of recurrence score in early breast cancer
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- Lien, Tonje G. (author)
- Oslo university hospital
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- Ohnstad, Hege Oma (author)
- Oslo university hospital
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- Lingjærde, Ole Christian (author)
- Oslo university hospital,University of Oslo
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- Vallon-Christersson, Johan (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Aaserud, Marit (author)
- Oslo university hospital
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- Sveli, My Anh Tu (author)
- Oslo university hospital
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- Borg, Åke (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Familjär bröstcancer,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Familial Breast Cancer,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Garred, Øystein (author)
- Oslo university hospital
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- Borgen, Elin (author)
- Oslo university hospital
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- Naume, Bjørn (author)
- Oslo university hospital,University of Oslo
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- Russnes, Hege (author)
- Oslo university hospital
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- Sørlie, Therese (author)
- University of Oslo,Oslo university hospital
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(creator_code:org_t)
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- 2021-12-04
- 2021
- English.
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In: Cancers. - : MDPI AG. - 2072-6694. ; 13:23
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Abstract
Subject headings
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- The PAM50 gene expression subtypes and the associated risk of recurrence (ROR) score are used to predict the risk of recurrence and the benefits of adjuvant therapy in early-stage breast cancer. The Prosigna assay includes the PAM50 subtypes along with their clinicopathological fea-tures, and is approved for treatment recommendations for adjuvant hormonal therapy and chemotherapy in hormone-receptor-positive early breast cancer. The Prosigna test utilizes RNA extracted from macrodissected tumor cells obtained from formalin-fixed, paraffin-embedded (FFPE) tissue sections. However, RNA extracted from fresh-frozen (FF) bulk tissue without macrodissection is widely used for research purposes, and yields high-quality RNA for downstream analyses. To in-vestigate the impact of the sample preparation approach on ROR scores, we analyzed 94 breast carcinomas included in an observational study that had available gene expression data from macro-dissected FFPE tissue and FF bulk tumor tissue, along with the clinically approved Prosigna scores for the node-negative, hormone-receptor-positive, HER2-negative cases (n = 54). ROR scores were calculated in R; the resulting two sets of scores from FFPE and FF samples were compared, and treatment recommendations were evaluated. Overall, ROR scores calculated based on the macro-dissected FFPE tissue were consistent with the Prosigna scores. However, analyses from bulk tissue yielded a higher proportion of cases classified as normal-like; these were samples with relatively low tumor cellularity, leading to lower ROR scores. When comparing ROR scores (low, intermedi-ate, and high), discordant cases between the two preparation approaches were revealed among the luminal tumors; the recommended treatment would have changed in a minority of cases.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Breast cancer
- Bulk
- FFPE
- Fresh-frozen
- Macrodissection
- PAM50
- Prosigna
- Risk of recurrence score
Publication and Content Type
- art (subject category)
- ref (subject category)
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Cancers
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To the university's database
- By the author/editor
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Lien, Tonje G.
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Ohnstad, Hege Om ...
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Lingjærde, Ole C ...
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Vallon-Christers ...
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Aaserud, Marit
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Sveli, My Anh Tu
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Borg, Åke
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Garred, Øystein
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Borgen, Elin
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Naume, Bjørn
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Russnes, Hege
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Sørlie, Therese
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Cancers
- By the university
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Lund University