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Lien, Tonje G.Oslo university hospital
(author)
Sample preparation approach influences pam50 risk of recurrence score in early breast cancer
- Article/chapterEnglish2021
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LIBRIS-ID:oai:lup.lub.lu.se:fac8f3a4-29d4-461b-b3e6-241877dbda13
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https://lup.lub.lu.se/record/fac8f3a4-29d4-461b-b3e6-241877dbda13URI
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https://doi.org/10.3390/cancers13236118DOI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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The PAM50 gene expression subtypes and the associated risk of recurrence (ROR) score are used to predict the risk of recurrence and the benefits of adjuvant therapy in early-stage breast cancer. The Prosigna assay includes the PAM50 subtypes along with their clinicopathological fea-tures, and is approved for treatment recommendations for adjuvant hormonal therapy and chemotherapy in hormone-receptor-positive early breast cancer. The Prosigna test utilizes RNA extracted from macrodissected tumor cells obtained from formalin-fixed, paraffin-embedded (FFPE) tissue sections. However, RNA extracted from fresh-frozen (FF) bulk tissue without macrodissection is widely used for research purposes, and yields high-quality RNA for downstream analyses. To in-vestigate the impact of the sample preparation approach on ROR scores, we analyzed 94 breast carcinomas included in an observational study that had available gene expression data from macro-dissected FFPE tissue and FF bulk tumor tissue, along with the clinically approved Prosigna scores for the node-negative, hormone-receptor-positive, HER2-negative cases (n = 54). ROR scores were calculated in R; the resulting two sets of scores from FFPE and FF samples were compared, and treatment recommendations were evaluated. Overall, ROR scores calculated based on the macro-dissected FFPE tissue were consistent with the Prosigna scores. However, analyses from bulk tissue yielded a higher proportion of cases classified as normal-like; these were samples with relatively low tumor cellularity, leading to lower ROR scores. When comparing ROR scores (low, intermedi-ate, and high), discordant cases between the two preparation approaches were revealed among the luminal tumors; the recommended treatment would have changed in a minority of cases.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Ohnstad, Hege OmaOslo university hospital
(author)
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Lingjærde, Ole ChristianOslo university hospital,University of Oslo
(author)
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Vallon-Christersson, JohanLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,LUCC: Lund University Cancer Centre,Other Strong Research Environments(Swepub:lu)onk-jvc
(author)
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Aaserud, MaritOslo university hospital
(author)
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Sveli, My Anh TuOslo university hospital
(author)
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Borg, ÅkeLund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Familjär bröstcancer,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Familial Breast Cancer,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments(Swepub:lu)onk-abo
(author)
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Garred, ØysteinOslo university hospital
(author)
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Borgen, ElinOslo university hospital
(author)
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Naume, BjørnOslo university hospital,University of Oslo
(author)
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Russnes, HegeOslo university hospital
(author)
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Sørlie, ThereseUniversity of Oslo,Oslo university hospital
(author)
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Oslo university hospitalUniversity of Oslo
(creator_code:org_t)
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on behalf of OSBREAC
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In:Cancers: MDPI AG13:232072-6694
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Lien, Tonje G.
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Ohnstad, Hege Om ...
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Lingjærde, Ole C ...
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Vallon-Christers ...
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Aaserud, Marit
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Sveli, My Anh Tu
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Borg, Åke
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Garred, Øystein
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Borgen, Elin
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Naume, Bjørn
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Russnes, Hege
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Sørlie, Therese
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- MEDICAL AND HEALTH SCIENCES
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Cancers
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