Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration

Bowler, Matthew W. (author)

Mueller, Uwe (author)

Weiss, Manfred S. (author)

Sanchez-Weatherby, Juan (author)

Sorensen, Thomas L-M. (author)

Thunnissen, Marjolein (author): Lund University,Lunds universitet,MAX IV-laboratoriet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,MAX IV Laboratory,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH

Ursby, Thomas (author): Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory

Gobbo, Alexandre (author)

Russi, Silvia (author)

Bowler, Michael G. (author)

Brockhauser, Sandor (author)

Svensson, Olof (author)

Cipriani, Florent (author)

(creator_code:org_t)

2015-01-29
2015
English.
In: Crystal Growth & Design. - : American Chemical Society (ACS). - 1528-7483 .- 1528-7505. ; 15:3, s. 1043-1054

Related links:: http://dx.doi.org/10...; show more...; https://doi.org/10.1...; https://lup.lub.lu.s...; https://doi.org/10.1...; show less...

Abstract Subject headings

Controlled dehydration of macromolecular crystals can lead to significant improvements in crystalline order, which often manifests itself in higher diffraction quality. Devices that can accurately control the humidity surrounding crystals on a beamline have led to this technique being increasingly adopted as experiments become easier and more reproducible. However, these experiments are often carried out by trial and error, and in order to facilitate and streamline them four European synchrotrons have established a collaboration around the HC1b dehydration device. The MAX IV Laboratory, Diamond Light Source, BESSY II, and the EMBL Grenoble Outstation/ESRF have pooled information gathered from user experiments, and on the use of the device, to propose a set of guidelines for these experiments. Here, we present the status and automation of the installations, advice on how best to perform experiments using the device, and an analysis of successful experiments that begins to show some trends in the type of protocols required by some systems. The dehydration methods shown are applicable to any device that allows control of the relative humidity of the air surrounding a macromolecular crystal.

By the author/editor: Bowler, Matthew ...; Mueller, Uwe; Weiss, Manfred S ...; Sanchez-Weatherb ...; Sorensen, Thomas ...; Thunnissen, Marj ...; show more...; Ursby, Thomas; Gobbo, Alexandre; Russi, Silvia; Bowler, Michael ...; Brockhauser, San ...; Svensson, Olof; Cipriani, Floren ...; show less...

About the subject

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