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Characterization of...
Characterization of folded recombinant Der p 5, a potential diagnostic marker allergen for house dust mite allergy
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Weghofer, M (author)
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Grote, M (author)
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Dall'Antonia, Y (author)
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Fernandez-Caldas, E (author)
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Krauth, MT (author)
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- van Hage, M (author)
- Karolinska Institutet
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Horak, F (author)
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Thomas, WR (author)
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Valent, P (author)
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Keller, W (author)
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Valenta, R (author)
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Vrtala, S (author)
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(creator_code:org_t)
- 2008-06-03
- 2008
- English.
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In: International archives of allergy and immunology. - : S. Karger AG. - 1423-0097 .- 1018-2438. ; 147:2, s. 101-109
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https://doi.org/10.1...
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Abstract
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- <i>Background:</i> Der p 5 was reported as an important allergen in<i> Dermatophagoides pteronyssinus</i>, which is particularly recognized by patients suffering from asthma. The aim of this study was to produce, by recombinant DNA technology, a folded Der p 5 allergen for diagnostic, therapeutic and preventive purposes. <i>Methods:</i> Der p 5-encoding cDNA was isolated from a λgt11 <i>D. pteronyssinus</i> expression cDNA library and expressed in <i>Escherichia coli</i>. rDer p 5 was purified to homogeneity and characterized by mass spectroscopy and circular dichroism. IgE reactivity was tested with sera from 117 mite-allergic patients and in a basophil histamine release experiment. Der p 5-specific rabbit IgG antibodies were produced for the ultrastructural localization of the allergen in mites by immunogold electron microscopy as well as for cross-reactivity studies. <i>Results:</i> rDer p 5 is a heat-stable protein with predominantly α-helical secondary structure which reacted with IgE from 31% of mite-allergic patients’ sera and showed no relevant cross-reactivity to group 5 allergens from storage mites and tropical mites. rDer p 5-specific rabbit IgG antibodies inhibited mite-allergic patients’ IgE binding to Der p 5 and allowed to localize the allergen in secretory granules of midgut epithelial cells of house dust mites. <i>Conclusions:</i> The described rDer p 5 molecule may be useful for diagnosis and immunotherapy of house dust mite-allergic patients.
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- By the author/editor
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Weghofer, M
-
Grote, M
-
Dall'Antonia, Y
-
Fernandez-Caldas ...
-
Krauth, MT
-
van Hage, M
-
show more...
-
Horak, F
-
Thomas, WR
-
Valent, P
-
Keller, W
-
Valenta, R
-
Vrtala, S
-
show less...
- Articles in the publication
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International ar ...
- By the university
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Karolinska Institutet