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Effects of palmitat...
Effects of palmitate on Ca(2+) handling in adult control and ob/ob cardiomyocytes: impact of mitochondrial reactive oxygen species
- Article/chapterEnglish2007
Publisher, publication year, extent ...
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American Diabetes Association,2007
Numbers
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:12128586
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http://kipublications.ki.se/Default.aspx?queryparsed=id:12128586URI
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https://doi.org/10.2337/db06-0739DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Obesity and insulin resistance are associated with enhanced fatty acid utilization, which may play a central role in diabetic cardiomyopathy. We now assess the effect of the saturated fatty acid palmitate (1.2 mmol/l) on Ca2+ handling, cell shortening, and mitochondrial production of reactive oxygen species (ROS) in freshly isolated ventricular cardiomyocytes from normal (wild-type) and obese, insulin-resistant ob/ob mice. Cardiomyocytes were electrically stimulated at 1 Hz, and the signal of fluorescent indicators was measured with confocal microscopy. Palmitate decreased the amplitude of cytosolic Ca2+ transients (measured with fluo-3), the sarcoplasmic reticulum Ca2+ load, and cell shortening by ∼20% in wild-type cardiomyocytes; these decreases were prevented by the general antioxidant N-acetylcysteine. In contrast, palmitate accelerated Ca2+ transients and increased cell shortening in ob/ob cardiomyocytes. Application of palmitate rapidly dissipated the mitochondrial membrane potential (measured with tetra-methyl rhodamine-ethyl ester) and increased the mitochondrial ROS production (measured with MitoSOX Red) in wild-type but not in ob/ob cardiomyocytes. In conclusion, increased saturated fatty acid levels impair cellular Ca2+ handling and contraction in a ROS-dependent manner in normal cardiomyocytes. Conversely, high fatty acid levels may be vital to sustain cardiac Ca2+ handling and contraction in obesity and insulin-resistant conditions.
Added entries (persons, corporate bodies, meetings, titles ...)
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Andersson, DCKarolinska Institutet
(author)
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Zhang, SJKarolinska Institutet
(author)
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Lanner, JTKarolinska Institutet
(author)
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Wibom, RKarolinska Institutet
(author)
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Katz, AKarolinska Institutet
(author)
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Bruton, JDKarolinska Institutet
(author)
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Westerblad, HKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Diabetes: American Diabetes Association56:4, s. 1136-11420012-17971939-327X
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