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Soluble HIV-1 Env t...
Soluble HIV-1 Env trimers in adjuvant elicit potent and diverse functional B cell responses in primates
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- Sundling, C (author)
- Karolinska Institutet
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- Forsell, MNE (author)
- Karolinska Institutet
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O'Dell, S (author)
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Feng, Y (author)
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Chakrabarti, B (author)
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Rao, SS (author)
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- Lore, K (author)
- Karolinska Institutet
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Mascola, JR (author)
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Wyatt, RT (author)
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- Douagi, I (author)
- Karolinska Institutet
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- Hedestam, GBK (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2010-08-02
- 2010
- English.
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In: The Journal of experimental medicine. - : Rockefeller University Press. - 1540-9538 .- 0022-1007. ; 207:9, s. 2003-2017
- Related links:
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http://jem.rupress.o...
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https://doi.org/10.1...
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Abstract
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- Broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoproteins (Envs) have proven difficult to elicit by immunization. Therefore, to identify effective Env neutralization targets, efforts are underway to define the specificities of bNAbs in chronically infected individuals. For a prophylactic vaccine, it is equally important to define the immunogenic properties of the heavily glycosylated Env in healthy primates devoid of confounding HIV-induced pathogenic factors. We used rhesus macaques to investigate the magnitude and kinetics of B cell responses stimulated by Env trimers in adjuvant. Robust Env-specific memory B cell responses and high titers of circulating antibodies developed after trimer inoculation. Subsequent immunizations resulted in significant expansion of Env-specific IgG-producing plasma cell populations and circulating Abs that displayed increasing avidity and neutralization capacity. The neutralizing activity elicited with the regimen used was, in most aspects, superior to that elicited by a regimen based on monomeric Env immunization in humans. Despite the potency and breadth of the trimer-elicited response, protection against heterologous rectal simian-HIV (SHIV) challenge was modest, illustrating the challenge of eliciting sufficient titers of cross-reactive protective NAbs in mucosal sites. These data provide important information for the design and evaluation of vaccines aimed at stimulating protective HIV-1 immune responses in humans.
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- art (subject category)
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- By the author/editor
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Sundling, C
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Forsell, MNE
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O'Dell, S
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Feng, Y
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Chakrabarti, B
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Rao, SS
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show more...
-
Lore, K
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Mascola, JR
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Wyatt, RT
-
Douagi, I
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Hedestam, GBK
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show less...
- Articles in the publication
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The Journal of e ...
- By the university
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Karolinska Institutet