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PARP1 promotes nucl...
PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1
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Pines, A (author)
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Vrouwe, MG (author)
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Marteijn, JA (author)
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Typas, D (author)
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Luijsterburg, MS (author)
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Cansoy, M (author)
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Hensbergen, P (author)
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Deelder, A (author)
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de Groot, A (author)
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Matsumoto, S (author)
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Sugasawa, K (author)
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Thoma, N (author)
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Vermeulen, W (author)
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Vrieling, H (author)
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Mullenders, L (author)
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- 2012-10-08
- 2012
- English.
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In: The Journal of cell biology. - : Rockefeller University Press. - 1540-8140 .- 0021-9525. ; 199:2, s. 235-249
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http://jcb.rupress.o...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- The WD40-repeat protein DDB2 is essential for efficient recognition and subsequent removal of ultraviolet (UV)-induced DNA lesions by nucleotide excision repair (NER). However, how DDB2 promotes NER in chromatin is poorly understood. Here, we identify poly(ADP-ribose) polymerase 1 (PARP1) as a novel DDB2-associated factor. We demonstrate that DDB2 facilitated poly(ADP-ribosyl)ation of UV-damaged chromatin through the activity of PARP1, resulting in the recruitment of the chromatin-remodeling enzyme ALC1. Depletion of ALC1 rendered cells sensitive to UV and impaired repair of UV-induced DNA lesions. Additionally, DDB2 itself was targeted by poly(ADP-ribosyl)ation, resulting in increased protein stability and a prolonged chromatin retention time. Our in vitro and in vivo data support a model in which poly(ADP-ribosyl)ation of DDB2 suppresses DDB2 ubiquitylation and outline a molecular mechanism for PARP1-mediated regulation of NER through DDB2 stabilization and recruitment of the chromatin remodeler ALC1.
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Pines, A
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Vrouwe, MG
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Marteijn, JA
-
Typas, D
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Luijsterburg, MS
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Cansoy, M
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show more...
-
Hensbergen, P
-
Deelder, A
-
de Groot, A
-
Matsumoto, S
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Sugasawa, K
-
Thoma, N
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Vermeulen, W
-
Vrieling, H
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Mullenders, L
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show less...
- Articles in the publication
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The Journal of c ...
- By the university
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Karolinska Institutet