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Chromosomal aneuplo...
Chromosomal aneuploidy affects the global proteome equilibrium of colorectal cancer cells
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Gemoll, T (författare)
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Habermann, JK (författare)
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Becker, S (författare)
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Szymczak, S (författare)
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Upender, MB (författare)
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Bruch, HP (författare)
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Hellman, U (författare)
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Ried, T (författare)
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- Auer, G (författare)
- Karolinska Institutet
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- Jornvall, H (författare)
- Karolinska Institutet
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Roblick, UJ (författare)
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(creator_code:org_t)
- Hindawi Limited, 2013
- 2013
- Engelska.
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Ingår i: Analytical cellular pathology (Amsterdam). - : Hindawi Limited. - 2210-7185 .- 2210-7177. ; 36:5-6, s. 149-161
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https://doi.org/10.1...
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Abstract
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- Background: Chromosomal aneuploidy has been identified as a prognostic factor in the majority of sporadic carcinomas. However, it is not known how chromosomal aneuploidy affects chromosome-specific protein expression in particular, and the cellular proteome equilibrium in general.Objective: The aim was to detect chromosomal aneuploidy-associated expression changes in cell clones carrying trisomies found in colorectal cancer.Methods: We used microcell-mediated chromosomal transfer to generate three artificial trisomic cell clones of the karyotypically stable, diploid, yet mismatch-deficient, colorectal cancer cell line DLD1 - each of them harboring one extra copy of either chromosome 3, 7 or 13. Protein expression differences were assessed by two-dimensional gel electrophoresis and mass spectrometry, compared to whole-genome gene expression data, and evaluated by PANTHER classification system and Ingenuity Pathway Analysis (IPA).Results: In total, 79 differentially expressed proteins were identified between the trisomic clones and the parental cell line. Up-regulation of PCNA and HMGB1 as well as down-regulation of IDH3A and PSMB3 were revealed as trisomy-associated alterations involved in regulating genome stability.Conclusions: These results show that trisomies affect the expression of genes and proteins that are not necessarily located on the trisomic chromosome, but reflect a pathway-related alteration of the cellular equilibrium.
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- Av författaren/redakt...
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Gemoll, T
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Habermann, JK
-
Becker, S
-
Szymczak, S
-
Upender, MB
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Bruch, HP
-
visa fler...
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Hellman, U
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Ried, T
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Auer, G
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Jornvall, H
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Roblick, UJ
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visa färre...
- Artiklar i publikationen
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Analytical cellu ...
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Karolinska Institutet